A rationally designed, pyrene-spiropyran hybrid Ca²⁺ sensor (Py-1) with enhanced fluorescence intensity compared to a standalone spiropyran analogue is presented. Importantly, Py-1 retains the characteristic red emission profile of the spiropyran, while fibre-based photostability studies show the sensor is stable after multiple cycles of photoswitching, without any sign of photodegradation. Such properties are of real advantage for cell-based sensing applications. An interesting observation is that, Py-1 presents with two excitation options; direct green excitation (532 nm) of the photoswitch for a red emission, and UV excitation (344 nm) of the component pyrene, which gives rise to distinct blue and red emissions. This proof-of-concept hybrid sensing system presents as a more general approach to brighter spiropyran-based sensors.

提出了一种合理设计的pyr-螺吡喃混合型Ca ²⁺传感器（Py-1），与单独的螺吡喃类似物相比，具有增强的荧光强度。重要的是，Py-1保留了螺吡喃的特征性红色发射曲线，而基于纤维的光稳定性研究表明，该传感器在经过多次光开关后仍保持稳定，没有任何光降解迹象。这样的性质对于基于细胞的感测应用具有真正的优势。有趣的发现是Py-1呈现两个激励选项；光电开关的直接绿色激发（532 nm）产生红色发射，而pyr组分的UV激发（344 nm）产生明显的蓝色和红色发射。这种概念验证的混合传感系统是更明亮的基于螺吡喃的传感器的通用方法。