Two rhodamine-based probes were designed and prepared, which exhibited highly sensitive and selective fluorescence enhancement upon binding to Pd²⁺ by UV–vis and fluorescence spectroscopies. Meanwhile the distinct color changes and rapid switch-on fluorescence also provided “naked-eyes” detection for Pd²⁺ over a broad pH range. The recognition mechanism was explored through Job’s plot, MS data, IR spectra and related theoretical calculations. Furthermore, the probes were applied for biological imaging to confirm that they can be used for monitoring Pd²⁺ in living cells (L929 and A549 cells) and living mice with satisfying results, which further demonstrated their value of practical applications in environmental and biological systems.

设计并制备了两种基于罗丹明的探针，它们在通过UV-vis和荧光光谱法与Pd ²⁺结合后表现出高度敏感和选择性的荧光增强。同时，明显的颜色变化和快速开启的荧光还提供了在宽pH范围内对Pd ^2+的“裸眼”检测。通过Job's图，MS数据，IR光谱和相关的理论计算来探索识别机制。此外，该探针用于生物成像，以证实它们可用于监测活细胞（L929和A549细胞）和活小鼠中的Pd ²⁺，并获得令人满意的结果，这进一步证明了它们在环境和生物系统中的实际应用价值。