An acoustic on-chip goniometer for room temperature macromolecular crystallography,Lab on a Chip

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An acoustic on-chip goniometer for room temperature macromolecular crystallography
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-11-01 00:00:00 , DOI: 10.1039/c7lc00812k
C. G. Burton _{1,

2,

3,

4} , D. Axford _{4,

5,

6,

7} , A. M. J. Edwards _{4,

8,

9,

10} , R. J. Gildea _{4,

5,

6,

7} , R. H. Morris _{4,

8,

9,

10} , M. I. Newton _{4,

8,

9,

10} , A. M. Orville _{4,

6,

7,

11} , M. Prince _{1,

2,

3,

4} , P. D. Topham _{1,

2,

3,

4} , P. T. Docker _{4,

5,

6,

7}

Affiliation

This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for gradual in-fluid sample reorientation about varying axes and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facilitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

中文翻译：

用于室温大分子晶体学的声学片上测角仪

本文介绍了通过声学感应旋转在室温下大分子晶体学中使用片上测角仪的设计，开发和成功使用。我们首次提出了一种低成本，速率可调的声波执行器，用于逐步改变流体样品在不同轴上的方向，并利用其在同步辐射束线上确定蛋白质的结构。该设备能够通过旋转方法从表面受限液滴内的样品中高效收集衍射数据。该方法有助于在流体环境中进行动力学研究的有效的大分子结构数据采集。

更新日期：2017-11-10

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