Micropatterning is a widely used powerful tool to create highly ordered microstructures on material surfaces. However, due to technical limitations, the integration of micropatterned microstructures into bioinspired 3D scaffolds to successfully regenerate well‐organized functional tissues is not achieved. In this work, a unique maskless micropatterning technology is reported to create 3D nanofibrous matrices with highly organized tubular architecture for tissue regeneration. This micropatterning method is a laser‐guided, noncontact, high‐precision, flexible computer programming of machining process that can create highly ordered tubules with the density ranged from 1000 to 60 000 mm⁻² and the size varied from 300 nm to 30 µm in the bioinspired 3D matrix. The tubular architecture presents pivotal biophysical cues to control dental pulp stem cell alignment, migration, polarization, and differentiation. More importantly, when using this 3D tubular hierarchical matrix as a scaffold, this study successfully regenerates functional tubular dentin that has the same well‐organized microstructure as its natural counterpart. This 3D maskless micropattern approach represents a powerful avenue not only for the exploration of cell–material interactions in 3D, but also for the regeneration of functional tissues with well‐organized microstructures.

中文翻译：

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用于高度组织化的管状组织再生的3D无掩模微图案设计

微图案化是一种广泛使用的强大工具，可以在材料表面上创建高度有序的微结构。但是，由于技术限制，无法将微图案化的微结构整合到受生物启发的3D支架中以成功再生组织良好的功能组织。在这项工作中，据报道独特的无掩模微图案化技术可创建具有高度组织化的管状结构的3D纳米纤维基质，用于组织再生。这种微图案化方法是激光引导，非接触式，高精度，灵活的加工过程计算机编程，可以创建密度从1000到60 000 mm ^-2的高度有序的小管。在受生物启发的3D矩阵中，尺寸从300 nm到30 µm不等。管状结构提供了关键的生物物理线索，以控制牙髓干细胞的排列，迁移，极化和分化。更重要的是，当将这种3D管状分层矩阵用作支架时，这项研究成功地再生了功能性管状牙本质，其微观结构与其天然对应物相同。这种3D无掩膜微模式方法不仅为探索3D中的细胞与材料的相互作用提供了一条强大的途径，而且为具有组织良好的微结构的功能组织的再生提供了一条强大的途径。