A nile red-based near-infrared fluorescent probe for endogenous hydrogen polysulfides in living cells,Analytical Methods

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A nile red-based near-infrared fluorescent probe for endogenous hydrogen polysulfides in living cells
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-25 00:00:00 , DOI: 10.1039/c7ay02376f
Kai-Bin Li _{1,

2,

3} , Feng-Zao Chen _{1,

2,

3} , Siqi Zhang _{1,

2,

3} , Wei Shi _{1,

2,

3} , De-Man Han _{1,

2,

3} , Chen Cai _{1,

2,

3} , Cai-Xia Chen _{1,

2,

3}

Affiliation

Endogenous hydrogen polysulfides (H₂S_n; n > 1) have attracted increasing attention because emerging studies have shown that it is actually the key signaling molecule rather than hydrogen sulfide (H₂S). Therefore, developing probes for selective and sensitive detection of hydrogen polysulfides is of great significance in biological science. In this study, a new fluorescent off–on probe for the selective and sensitive detection of hydrogen polysulfides (H₂S_n) with near-infrared (NIR) fluorescent signal change has been designed and synthesized. The probe was synthesized by an esterification reaction coupling between a nile red dye and a bis-electrophilic H₂S_n capture group 2-fluoro-5-nitrobenzoic acid. The probe showed a specific off–on response to H₂S_n in aqueous solution with nanomolar LOD without interference by a range of competing sulfocompounds and several reactive nitrogen and oxygen species. Moreover, it can be used for imaging of endogenous H₂S_n in living cells.

中文翻译：

基于尼罗红的近红外荧光探针，用于活细胞中的内源性多硫化氢

内源性多硫化氢（H ₂ S _n；n > 1）已引起越来越多的关注，因为新兴研究表明，它实际上是关键的信号分子，而不是硫化氢（H ₂ S）。因此，开发用于选择性和灵敏检测多硫化氢的探针在生物科学中具有重要意义。在这项研究中，已经设计并合成了一种新型的荧光关闭探针，用于选择性和灵敏地检测具有近红外（NIR）荧光信号变化的多硫化氢（H ₂ S _n）。该探针通过尼罗红染料和双亲电子H ₂ S之间的酯化反应偶联而合成_n捕获基团2-氟-5-硝基苯甲酸。该探针显示出对具有纳摩尔级LOD的水溶液中H₂ S_n的特定的开-关响应，而不受一系列竞争性磺化合物和几种反应性氮和氧物种的干扰。此外，它可用于活细胞中内源性H₂ S_n的成像。

更新日期：2017-11-09

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