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SERS-fluorescence bimodal nanoprobes for in vitro imaging of the fatty acid responsive receptor GPR120
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-18 00:00:00 , DOI: 10.1039/c7ay02039b Lifu Xiao 1, 2, 3, 4, 5 , Abdul K. Parchur 1, 2, 3, 4 , Timothy A. Gilbertson 2, 3, 4, 6 , Anhong Zhou 1, 2, 3, 4
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-18 00:00:00 , DOI: 10.1039/c7ay02039b Lifu Xiao 1, 2, 3, 4, 5 , Abdul K. Parchur 1, 2, 3, 4 , Timothy A. Gilbertson 2, 3, 4, 6 , Anhong Zhou 1, 2, 3, 4
Affiliation
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G-protein-coupled receptor 120 (GPR120), as a member of the rhodopsin family of G-protein-coupled receptors, has been shown to function as a sensor for dietary fat in the gustatory and digestive systems. Its specific role in the chemoreception of fatty acids, which is thought to be crucial in understanding the mechanism surrounding the control of fat intake and, accordingly, in the treatment of obesity, remains unclear. Here we report a novel surface-enhanced Raman spectroscopy (SERS)-fluorescence bimodal microscopic technique for detection and imaging of GPR120 in single living cells. CaMoO4:Eu3+@AuNR hybrid nanoparticles are synthesized and characterized as imaging probes. The biocompatibility and imaging capability of the probes are investigated using a model HEK293 cell line with an inducible GPR120 gene transfection. The cellular distribution of GPR120 is visualized by single-cell SERS and fluorescence imaging. A dose-dependent GPR120 response to linoleic acid treatment is revealed by SERS.
中文翻译：
SERS荧光双峰纳米探针用于脂肪酸响应受体GPR120的体外成像
G蛋白偶联受体120（GPR120）是视紫红质G蛋白偶联受体家族的成员，已被证明在味觉和消化系统中可作为膳食脂肪的传感器。目前尚不清楚其在脂肪酸化学感受中的特定作用，这对于理解控制脂肪摄入的机制至关重要，因此在肥胖症的治疗中也至关重要。在这里，我们报告一种新型的表面增强拉曼光谱（SERS）-荧光双峰显微镜技术，用于检测和成像单个活细胞中的GPR120。CaMoO 4：Eu 3+合成了@AuNR杂化纳米颗粒并表征为成像探针。使用具有可诱导的GPR120基因转染的模型HEK293细胞系，研究了探针的生物相容性和成像能力。通过单细胞SERS和荧光成像可以观察到GPR120的细胞分布。SERS揭示了对亚油酸治疗的剂量依赖性GPR120反应。
更新日期：2017-11-09
中文翻译：
SERS荧光双峰纳米探针用于脂肪酸响应受体GPR120的体外成像
G蛋白偶联受体120（GPR120）是视紫红质G蛋白偶联受体家族的成员，已被证明在味觉和消化系统中可作为膳食脂肪的传感器。目前尚不清楚其在脂肪酸化学感受中的特定作用，这对于理解控制脂肪摄入的机制至关重要，因此在肥胖症的治疗中也至关重要。在这里，我们报告一种新型的表面增强拉曼光谱（SERS）-荧光双峰显微镜技术，用于检测和成像单个活细胞中的GPR120。CaMoO 4：Eu 3+合成了@AuNR杂化纳米颗粒并表征为成像探针。使用具有可诱导的GPR120基因转染的模型HEK293细胞系，研究了探针的生物相容性和成像能力。通过单细胞SERS和荧光成像可以观察到GPR120的细胞分布。SERS揭示了对亚油酸治疗的剂量依赖性GPR120反应。
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