Nanowire sensors monitor bacterial growth kinetics and response to antibiotics,Lab on a Chip

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Nanowire sensors monitor bacterial growth kinetics and response to antibiotics
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-19 00:00:00 , DOI: 10.1039/c7lc00807d
B. Ibarlucea _{1,

2,

3,

4,

5} , T. Rim _{6,

7,

8,

9} , C. K. Baek _{6,

7,

8,

9} , J. A. G. M. de Visser _{10,

11,

12,

13} , L. Baraban _{1,

2,

3,

4,

5} , G. Cuniberti _{1,

2,

3,

4,

5}

Affiliation

Miniaturized and cost-efficient methods aiming at high throughput analysis of microbes are of great importance for the surveillance and control of infectious diseases and the related issue of antimicrobial resistance. Here we demonstrate a miniature nanosensor based on a honeycomb-patterned silicon nanowire field effect transistor (FET) capable of detection of bacterial growth and antibiotic response in microbiologically relevant nutrient media. We determine the growth kinetics and metabolic state of Escherichia coli cells in undiluted media via the quantification of changes in the source–drain current caused by varying pH values. Furthermore, by measuring the time dependent profile of pH change for bacterial cultures treated with antibiotics, we demonstrate for the first time the possibility of electrically distinguishing between bacteriostatic and bactericidal drug effects. We believe that the use of such nanoscopic FET devices enables addressing parameters that are not easily accessible by conventional optical methods in a label-free format, i.e. monitoring of microbial metabolic activity or stress response.

中文翻译：

纳米线传感器可监测细菌生长动力学和对抗生素的反应

旨在对微生物进行高通量分析的小型且经济高效的方法对于监测和控制传染病以及相关的耐药性问题具有重要意义。在这里，我们演示了一种基于蜂窝状硅纳米线场效应晶体管（FET）的微型纳米传感器，该晶体管能够检测微生物相关营养培养基中的细菌生长和抗生素反应。我们确定的生长动力学和代谢状态大肠杆菌在未稀释的培养基中的细胞通过pH值变化引起的源漏电流变化的量化。此外，通过测量用抗生素处理的细菌培养物的pH值随时间变化的曲线，我们首次证明了电学区分抑菌和杀菌药物作用的可能性。我们相信，使用这种纳米级FET器件可以解决传统光学方法不容易以无标签格式访问的参数，即监测微生物的代谢活性或应激反应。

更新日期：2017-11-09

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