How Bound and Free Fatty Acids in Cellulose Films Impact Nonspecific Protein Adsorption,Biomacromolecules

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How Bound and Free Fatty Acids in Cellulose Films Impact Nonspecific Protein Adsorption
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-11-08 00:00:00 , DOI: 10.1021/acs.biomac.7b01260
Katrin Niegelhell , Michael Süßenbacher , Jürgen Sattelkow , Harald Plank , Yonggui Wang ₁ , Kai Zhang ₁ , Stefan Spirk

Affiliation

The effect of fatty acids and fatty acid esters to impair nonspecific protein adsorption on cellulose thin films is investigated. Thin films are prepared by blending trimethylsilyl cellulose solutions with either cellulose stearoyl ester or stearic acid at various ratios. After film formation by spin coating, the trimethylsilyl cellulose fraction of the films is converted to cellulose by exposure to HCl vapors. The morphologies and surface roughness of the blends were examined by atomic force microscopy revealing different feature shapes and sizes depending on the blend ratios. Nonspecific protein adsorption at the example of bovine serum albumin toward the blend thin films was tested by means of surface plasmon resonance spectroscopy in real-time. Incorporation of stearic acid into the cellulose leads to highly protein repellent surfaces regardless of the amount added. The stearic acid acts as a sacrificial compound that builds a complex with bovine serum albumin thereby inhibiting protein adsorption. For the blends where stearoyl ester is added to the cellulose films, the cellulose:cellulose stearoyl ester ratios of 3:1 and 1:1 lead to much lower nonspecific protein adsorption compared to pure cellulose, whereas for the other ratios, adsorption increases. Supplementary results were obtained from atomic force microscopy experiments performed in liquid during exposure to protein solution and surface free energy determinations.

中文翻译：

纤维素膜中的结合脂肪酸和游离脂肪酸如何影响非特异性蛋白质吸附

研究了脂肪酸和脂肪酸酯削弱非特异性蛋白质在纤维素薄膜上的吸附作用。通过将三甲基硅烷基纤维素溶液与纤维素硬脂酰酯或硬脂酸以各种比例混合来制备薄膜。在通过旋涂形成膜之后，通过暴露于HCl蒸气将膜的三甲基硅烷基纤维素级分转化为纤维素。通过原子力显微镜检查共混物的形态和表面粗糙度，显示出取决于共混物比率的不同特征形状和尺寸。通过表面等离振子共振光谱法实时测试了牛血清白蛋白对混合薄膜的非特异性蛋白吸附。硬脂酸掺入纤维素中会导致高度排斥蛋白质的表面，而不管其添加量如何。硬脂酸作为一种牺牲化合物，与牛血清白蛋白形成复合物，从而抑制蛋白质的吸附。对于将硬脂酰酯添加到纤维素膜中的混合物，与纯纤维素相比，纤维素：纤维素硬脂酸酯的比率为3：1和1：1导致非特异性蛋白质的吸附要低得多，而对于其他比率，则吸附会增加。补充结果是通过原子力显微镜实验在蛋白质溶液中接触液体和表面自由能测定期间获得的。对于将硬脂酰酯添加到纤维素膜中的混合物，与纯纤维素相比，纤维素：纤维素硬脂酸酯的比率为3：1和1：1导致非特异性蛋白质的吸附要低得多，而对于其他比率，则吸附会增加。补充结果是通过原子力显微镜实验在蛋白质溶液中接触液体和表面自由能测定期间获得的。对于将硬脂酰酯添加到纤维素膜中的掺混物，与纯纤维素相比，纤维素：纤维素硬脂酰酯比率为3：1和1：1导致非特异性蛋白质吸附低得多，而对于其他比率，则吸附增加。补充结果是通过原子力显微镜实验在蛋白质溶液中接触液体和表面自由能测定期间获得的。

更新日期：2017-11-08

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