Immobilization of β-Cyclodextrin-Conjugated Lactoferrin onto Polymer Monolith for Enrichment of Ga in Metabolic Residues of Ga-Based Anticancer Drugs,Biomacromolecules

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Immobilization of β-Cyclodextrin-Conjugated Lactoferrin onto Polymer Monolith for Enrichment of Ga in Metabolic Residues of Ga-Based Anticancer Drugs
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acs.biomac.7b01003
Haijiao Zheng ₁ , Tenggao Zhu ₁ , Xiqian Li ₂ , Guan Wang ₃ , Qiong Jia ₁

Affiliation

Biological-material-functionalized porous monoliths were prepared with lactoferrin and β-cyclodextrin via a click reaction. With the monolith as an extraction medium, a method combined with ICP-MS was developed for the determination of total gallium originating from metabolic residues of orally bioavailable gallium complexes with tris(8-quinolinolato)gallium (GaQ₃) as a representative. The method exhibited favorable adsorption behaviors for gallium with high selectivity, low detection limit (2 ng L^–1), and an enrichment factor of 29-fold with the sample throughput of 30 min^–1. The developed approach was validated by the analysis of gallium from GaQ₃ metabolic residues in a human cell line. Additionally, the practical applicability of this method was evaluated by the determination of gallium in human blood and urine samples from cancer patients. Results illustrated that the prepared monolith had potential in Ga-based anticancer drug analysis in complex biological samples.

中文翻译：

将β-环糊精缀合的乳铁蛋白固定在聚合物整料上，以富集Ga基抗癌药物代谢残基中的Ga

通过点击反应，用乳铁蛋白和β-环糊精制备了生物材料功能化的多孔整体材料。以整料为提取介质，开发了一种以ICP-MS为基础的方法，用于测定以三（8-喹啉基）镓（GaQ ₃）为代表的口服生物利用镓复合物代谢残留物中的总镓。该方法显示出对镓的良好吸附行为，具有高选择性，低检测限（2 ng L ^–1）和富集因子29倍，样品通量为30 min ^–1。通过分析GaQ _3中的镓验证了开发的方法人细胞系中的代谢残基。另外，通过测定癌症患者的人血和尿液样品中的镓来评估该方法的实际适用性。结果表明，所制备的整体料在复杂生物样品中基于Ga的抗癌药物分析中具有潜力。

更新日期：2017-11-08

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