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Fabrication of Uniform Casein/CaCO3 Vaterite Microspheres and Investigation of Its Formation Mechanism
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acs.cgd.7b00306 Yan Li , Ximei Li , Zhinan Cao , Yue Xu , Yihong Gong , Xuetao Shi 1
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1021/acs.cgd.7b00306 Yan Li , Ximei Li , Zhinan Cao , Yue Xu , Yihong Gong , Xuetao Shi 1
Affiliation
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Uniform Casein/CaCO3 microspheres were fabricated with well tuned properties and the formation mechanism based on the templating effect of casein micelles was proposed. In bone tissue engineering, vaterite microspheres were promising because of the biocompatibility and easy fabrication. Here Casein/CaCO3 microspheres were precipitated by mixing CaCl2 and Na2CO3 solutions under stirring in the presence of casein. All samples were mainly in vaterite, composed of aggregates of nanosized crystals. With the increase of casein concentration, the amount of calcite and microsphere size decreased while the loading content of casein increased, suggesting that casein induced the formation of vaterite and also stabilized the crystal phase. The formation mechanism was further investigated. With the increase of CaCl2 amount, the size of the forming microspheres increased while the zeta potential was stabilized, the polycrystalline nature was shown, and the presence of Ca inside microspheres was confirmed. Hence, the formation mechanism based on casein micelles which demonstrated the template effect was proposed. Casein/CaCO3 microspheres enhanced the deposition of hydroxyapatite crystals and sample II-8 was tested as the most cytocompatible on mesenchymal stem cells. The properties of Casein/CaCO3 microspheres could be well controlled; for better performance in bone tissue engineering, formation of composite scaffolds may be preferred.
中文翻译：
均匀酪蛋白/ CaCO 3球ate石微球的制备及其形成机理的研究
制备了具有良好性能的均匀酪蛋白/ CaCO 3微球，并基于酪蛋白胶束的模板作用，提出了形成机理。在骨组织工程中，球ate石微球由于其生物相容性和易于制造而很有前途。通过混合CaCl 2和Na 2 CO 3沉淀酪蛋白/ CaCO 3微球酪蛋白存在下在搅拌下形成溶液。所有样品主要在球v石中，由纳米级晶体的聚集体组成。随着酪蛋白浓度的增加，方解石的数量和微球尺寸减小，而酪蛋白的负载量增加，这表明酪蛋白诱导了球ate石的形成并稳定了晶相。进一步研究了形成机理。随着CaCl 2量的增加，形成的微球的尺寸增加，同时ζ电势稳定，显示出多晶性质，并且证实了Ca在微球内部的存在。因此，提出了基于酪蛋白胶束的形成机理，其证明了模板作用。酪蛋白/ CaCO 3微球增强了羟基磷灰石晶体的沉积，样品II-8被测试为在间充质干细胞上具有最强的细胞相容性。酪蛋白/ CaCO 3微球的性质可以很好地控制；为了在骨组织工程中获得更好的性能，复合支架的形成可能是优选的。
更新日期：2017-11-08
中文翻译：
均匀酪蛋白/ CaCO 3球ate石微球的制备及其形成机理的研究
制备了具有良好性能的均匀酪蛋白/ CaCO 3微球，并基于酪蛋白胶束的模板作用，提出了形成机理。在骨组织工程中，球ate石微球由于其生物相容性和易于制造而很有前途。通过混合CaCl 2和Na 2 CO 3沉淀酪蛋白/ CaCO 3微球酪蛋白存在下在搅拌下形成溶液。所有样品主要在球v石中，由纳米级晶体的聚集体组成。随着酪蛋白浓度的增加，方解石的数量和微球尺寸减小，而酪蛋白的负载量增加，这表明酪蛋白诱导了球ate石的形成并稳定了晶相。进一步研究了形成机理。随着CaCl 2量的增加，形成的微球的尺寸增加，同时ζ电势稳定，显示出多晶性质，并且证实了Ca在微球内部的存在。因此，提出了基于酪蛋白胶束的形成机理，其证明了模板作用。酪蛋白/ CaCO 3微球增强了羟基磷灰石晶体的沉积，样品II-8被测试为在间充质干细胞上具有最强的细胞相容性。酪蛋白/ CaCO 3微球的性质可以很好地控制；为了在骨组织工程中获得更好的性能，复合支架的形成可能是优选的。
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