Lactofen - Electrochemical Sensing and Interaction with dsDNA,Electroanalysis

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Lactofen - Electrochemical Sensing and Interaction with dsDNA
Electroanalysis ( IF 2.7 ) Pub Date : 2017-11-08 , DOI: 10.1002/elan.201700472
Dariusz Guziejewski ₁ , Kamila Morawska ₁ , Tomasz Popławski ₂ , Radovan Metelka ₃ , Witold Ciesielski ₁ , Sylwia Smarzewska ₁

Affiliation

The electrochemical reduction of lactofen (LCT) at the glassy carbon (GCE) and silver amalgam film electrode (AMFE) is investigatedin detail by the means of square wave voltammetry (SWV), square wave stripping voltammetry (SWSV) and cyclic voltammetry. The influence of various factors such as supporting electrolyte composition and SW parameters were studied. The AMFE electrode showed an excellent electrochemical activity toward the electro-reduction of LCT, leading to a significant improvement in sensitivity as compared to the glassy carbon electrode.The SWSV detection limits for GCE and AMFE were 285.0 nM and 2.0 nM, respectively. The applicability of the developed voltammetric method for analysis of tap water and river water is illustrated with spiked samples analysis. Moreover, as lactofen is highly toxic to fish and other aquatic organisms, its interaction with dsDNA isolated from salmon sperm was tested. The intercalative mode of LCT binding to dsDNA was estimated. The heterogeneous rate constants were calculated for the free LCT and the LCT-dsDNA complex. Moreover, LCT-dsDNA complex binding ratio and equilibrium constant were determined. The decrease in the SWV peak current of LCT in the presence of dsDNA was used for the determination of dsDNA.

中文翻译：

Lactofen - 电化学传感和与 dsDNA 的相互作用

通过方波伏安法(SWV)、方波溶出伏安法(SWSV)和循环伏安法详细研究了乳酚(LCT)在玻璃碳(GCE)和银汞合金膜电极(AMFE)上的电化学还原。研究了各种因素的影响，如支持电解质组成和 SW 参数。AMFE 电极对 LCT 的电还原表现出优异的电化学活性，与玻碳电极相比，灵敏度显着提高。 GCE 和 AMFE 的 SWSV 检测限分别为 285.0 nM 和 2.0 nM。通过加标样品分析说明了所开发的伏安法在分析自来水和河水方面的适用性。此外，由于乳酚对鱼类和其他水生生物有剧毒，测试了它与从鲑鱼精子中分离的 dsDNA 的相互作用。估计了 LCT 与 dsDNA 结合的插入模式。计算游离 LCT 和 LCT-dsDNA 复合物的异质速率常数。此外，还确定了 LCT-dsDNA 复合物结合率和平衡常数。在 dsDNA 存在下 LCT 的 SWV 峰值电流的降低用于测定 dsDNA。

更新日期：2017-11-08

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