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A microfluidic design to provide a stable and uniform in vitro microenvironment for cell culture inspired by the redundancy characteristic of leaf areoles
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-11 00:00:00 , DOI: 10.1039/c7lc00343a Jingmin Li 1, 2, 3, 4 , Juan Wei 1, 2, 3, 4 , Yuanchang Liu 5, 6, 7, 8 , Bo Liu 2, 3, 4, 9 , Tao Liu 1, 2, 3, 4 , Yang Jiang 1, 2, 3, 4 , Laiqian Ding 1, 2, 3, 4 , Chong Liu 1, 2, 3, 4, 10
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-11 00:00:00 , DOI: 10.1039/c7lc00343a Jingmin Li 1, 2, 3, 4 , Juan Wei 1, 2, 3, 4 , Yuanchang Liu 5, 6, 7, 8 , Bo Liu 2, 3, 4, 9 , Tao Liu 1, 2, 3, 4 , Yang Jiang 1, 2, 3, 4 , Laiqian Ding 1, 2, 3, 4 , Chong Liu 1, 2, 3, 4, 10
Affiliation
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The leaf venation is considered to be an optimal transportation system with the mesophyll cells being divided by minor veins into small regions named areoles. The transpiration of water in different regions of a leaf fluctuates over time making the transportation of water in veins fluctuate as well. However, because of the existence of multiple paths provided by the leaf venation network and the pits on the walls of the vessels, the pressure field and nutrient concentration in the areoles that the mesophyll cells live in are almost uniform. Therefore, inspired by such structures, a microfluidic design of a novel cell culture chamber has been proposed to obtain a stable and uniform microenvironment. The device consists of a novel microchannel system imitating the vessels in the leaf venation to transport the culture medium, a cell culture chamber imitating the areole and microgaps imitating the pits. The effects of the areole and pit on flow fields in the cell culture chamber have been discussed. The results indicate that the bio-inspired microfluidic device is a robust platform to provide an in vivo like fluidic microenvironment.
中文翻译：
一种微流控设计，可为细胞培养提供稳定且均匀的体外微环境，其灵感来自于叶油菜豆的冗余特性
叶脉被认为是最佳的运输系统，叶肉细胞被次要静脉划分为小区域，称为小孔。随着时间的流逝，叶片不同区域的水蒸腾量也随之波动，从而使静脉中的水运输也随之波动。但是，由于叶片通气网络和血管壁上的凹坑提供了多条路径，因此叶肉细胞所生活的沙丘中的压力场和养分浓度几乎是均匀的。因此，受这种结构的启发，已经提出了新颖的细胞培养室的微流体设计以获得稳定且均匀的微环境。该设备由新型微通道系统组成，该系统模仿叶脉中的血管来运输培养基，模仿虹膜的细胞培养室和模仿凹坑的微间隙。讨论了槟榔和基坑对细胞培养室中流场的影响。结果表明，受生物启发的微流控设备是一个强大的平台，可提供体内就像流体微环境。
更新日期：2017-11-07
中文翻译：
一种微流控设计，可为细胞培养提供稳定且均匀的体外微环境，其灵感来自于叶油菜豆的冗余特性
叶脉被认为是最佳的运输系统，叶肉细胞被次要静脉划分为小区域，称为小孔。随着时间的流逝，叶片不同区域的水蒸腾量也随之波动，从而使静脉中的水运输也随之波动。但是，由于叶片通气网络和血管壁上的凹坑提供了多条路径，因此叶肉细胞所生活的沙丘中的压力场和养分浓度几乎是均匀的。因此，受这种结构的启发，已经提出了新颖的细胞培养室的微流体设计以获得稳定且均匀的微环境。该设备由新型微通道系统组成，该系统模仿叶脉中的血管来运输培养基，模仿虹膜的细胞培养室和模仿凹坑的微间隙。讨论了槟榔和基坑对细胞培养室中流场的影响。结果表明，受生物启发的微流控设备是一个强大的平台，可提供体内就像流体微环境。
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