Sustainable production of medium chain fatty acids (MCFA) with an enriched mixed bacterial culture: microbial characterization using molecular methods,Sustainable Energy & Fuels

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Sustainable production of medium chain fatty acids (MCFA) with an enriched mixed bacterial culture: microbial characterization using molecular methods
Sustainable Energy & Fuels ( IF 5.0 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7se00467b
M. Venkateswar Reddy _{1,

2,

3,

4,

5} , S. Venkata Mohan _{6,

7,

8,

9} , Young-Cheol Chang _{1,

2,

3,

4,

5}

Affiliation

Chain elongation is the process by which bacteria convert ethanol and short chain fatty acids (SCFA) into medium chain fatty acids (MCFA). In the present study, a bacterial mixed culture was enriched and used together with synthetic waste (SW) to produce commercially valuable SCFA and MCFA for 91 days by anaerobic fermentation. For the first time, the effects of substrate, electron donor, and methane inhibition on MCFA production were evaluated. The produced SCFA, MCFA and biogas were analyzed using chromatography techniques. Butyrate (C4) and caproate (C6) were the dominant products in SCFA and MCFA, respectively. Bacteria in conditions of methane inhibition produced the highest concentration of butyrate (8.2 g l⁻¹), caproate (8.6 g l⁻¹), heptanoate (0.7 g l⁻¹), and caprylate (0.23 g l⁻¹). The dominance of Clostridia, Rumen bacterium, and the Actinomyces group in the enriched culture were identified by denaturing gradient gel electrophoresis (DGGE) analysis. Studies are being conducted under pipeline with enriched cultures to produce SCFA and MCFA using the real waste streams.

中文翻译：

通过丰富的混合细菌培养物可持续生产中链脂肪酸（MCFA）：使用分子方法进行微生物鉴定

链延长是细菌将乙醇和短链脂肪酸（SCFA）转化为中链脂肪酸（MCFA）的过程。在本研究中，细菌混合培养物得到了富集，并与合成废料（SW）一起使用，通过厌氧发酵在91天内生产出具有商业价值的SCFA和MCFA。首次评估了底物，电子给体和甲烷抑制对MCFA产生的影响。使用色谱技术分析了产生的SCFA，MCFA和沼气。丁酸酯（C4）和己酸酯（C6）分别是SCFA和MCFA中的主要产品。甲烷抑制条件下的细菌产生最高浓度的丁酸酯（8.2 gl ^-1），己酸酯（8.6 gl ^-1），庚酸酯（0.7 gl ^-1）），然后辛酸（0.23 gl ^-1）。的主导地位梭菌，瘤胃细菌和放线菌的富集培养组通过变性梯度凝胶电泳（DGGE）分析鉴定。正在利用丰富的文化进行管道研究，以利用真实的废物流生产SCFA和MCFA。

更新日期：2017-11-06

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