A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells,Lab on a Chip

当前位置： X-MOL 学术 › Lab Chip › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-10-27 00:00:00 , DOI: 10.1039/c7lc00925a
Hyungseok Cho _{1,

2,

3,

4,

5} , Jinho Kim _{1,

2,

3,

4,

5} , Chang-Wan Jeon _{5,

6,

7,

8,

9} , Ki-Ho Han _{1,

2,

3,

4,

5}

Affiliation

We describe an assembly-disposable microfluidic device based on a silicone-coated release polymer thin film. It consists of a disposable polymeric superstrate and a reusable functional substrate and they are assembled simply using vacuum pressure. The disposable polymeric superstrate is manufactured by bonding a silicone-coated release polymer thin film and a microstructured polydimethylsiloxane (PDMS) replica, containing only a simple structured microchannel. The reusable functional substrate generates an intricate energy field that can penetrate the micrometer-thick polymer film into the microchannel and control microfluids. This is the first report to introduce a silicone-coated release polyethylene terephthalate (PET) thin film as a bonding layer on a microstructured PDMS replica. The bonding strength was ∼600 kPa, which is the strongest among bonding methods of PDMS and PET polymer. Additionally, accelerated tests for bond stability and leakage demonstrated that the silicone-coated release PET film can form a very robust bond with PDMS. To demonstrate the usefulness of the proposed assembly-disposable microfluidic device, a lateral magnetophoretic microseparator was developed in an assembly-disposable microfluidic device format and was evaluated for isolating circulating tumor cells (CTCs) from patients with breast cancer.

中文翻译：

具有可重复使用的磁热功能基质的一次性微流控装置，用于分离循环肿瘤细胞

我们描述了基于有机硅涂层的剥离聚合物薄膜的组装一次性微流控设备。它由一次性聚合物覆盖层和可重复使用的功能性衬底组成，并且只需使用真空压力即可组装。通过将有机硅涂层的剥离聚合物薄膜和仅包含简单结构化微通道的微结构化聚二甲基硅氧烷（PDMS）复制品粘合在一起，即可制成一次性聚合物覆板。可重复使用的功能性基材会产生复杂的能量场，该能量场可以穿透微米级的聚合物膜进入微通道并控制微流体。这是首次在微结构化PDMS复制品上引入有机硅涂层的聚对苯二甲酸乙二醇酯（PET）薄膜作为粘结层的报告。粘结强度约为600 kPa，这是PDMS和PET聚合物粘结方法中最强的。此外，加速的粘合稳定性和泄漏测试表明，涂有有机硅的离型PET膜可与PDMS形成非常牢固的粘合。为了证明所提出的一次性组装的微流体装置的实用性，开发了一种一次性组装的微流体装置形式的侧磁微分离器，并对其从乳腺癌患者中分离循环肿瘤细胞（CTC）进行了评估。

更新日期：2017-11-02

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11