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A label-free and enzyme-free platform with a visible output for constructing versatile logic gates using caged G-quadruplex as the signal transducer
Chemical Science ( IF 7.6 ) Pub Date : 2017-10-20 00:00:00 , DOI: 10.1039/c7sc04007e Junhua Chen 1, 2, 3, 4 , Jiafeng Pan 1, 2, 3, 4 , Shu Chen 1, 2, 3, 4
Chemical Science ( IF 7.6 ) Pub Date : 2017-10-20 00:00:00 , DOI: 10.1039/c7sc04007e Junhua Chen 1, 2, 3, 4 , Jiafeng Pan 1, 2, 3, 4 , Shu Chen 1, 2, 3, 4
Affiliation
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A complete set of binary basic logic gates (OR, AND, NOR, NAND, INHIBT, IMPLICATION, XOR and XNOR) is realized on a label-free and enzyme-free sensing platform using caged G-quadruplex as the signal transducer. In the presence of an appropriate input, the temporarily blocked G-rich sequence in the hairpin DNA is released through cleavage by the synergetically-stabilized Mg2+-dependent DNAzyme which can be made to function via the input-guided cooperative conjunction of the DNAzyme subunits. In the presence of hemin, the unblocked G-quadruplex DNAzyme catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 to generate a colored readout signal which can be readily distinguished by the naked eye. This strategy is quite versatile and straightforward for logic operations. Two combinatorial gates (XOR + AND and XOR + NOR) are also successfully fabricated to demonstrate the modularity and scalability of the computing elements. The distinctive advantage of this logic system is that molecular events in aqueous solution could be translated into a color change which can be directly observed by the naked eye without resorting to any analytical instrumentation. Moreover, this work reveals a new route for the design of molecular logic gates that can be executed without any labeling and immobilization procedure or separation and washing step, which holds great promise for intelligent point-of-care diagnostics and in-field applications.
中文翻译：
无标签和无酶平台，具有可见输出，可使用笼状G四联体作为信号转换器来构建多功能逻辑门
使用笼状G四联体作为信号传感器，在无标记和无酶的传感平台上实现了一套完整的二进制基本逻辑门（“或”，“与非”，“与非”，“隐含”，“异或”，“异或”）。在适当的输入的存在，所述临时阻塞富含G的发夹DNA序列通过裂解由协同地稳定释放的Mg 2+ -依赖性DNA核酶，其可以使功能通过DNA核酶的输入引导的协同结合亚单位。在存在血红素的情况下，未封闭的G-四链体DNAzyme催化H 2 O 2氧化3,3'，5,5'-四甲基联苯胺（TMB）产生彩色的读出信号，该信号可以用肉眼很容易地分辨出来。对于逻辑运算，此策略非常通用且简单。还成功制造了两个组合门（XOR + AND和XOR + NOR），以演示计算元素的模块化和可扩展性。该逻辑系统的独特优势在于，水溶液中的分子事件可以转化为颜色变化，而无需借助任何分析仪器，就可以通过肉眼直接观察到颜色变化。而且，这项工作揭示了分子逻辑门设计的新途径，该方法无需任何标记和固定程序或分离和洗涤步骤即可执行，这为智能即时诊断和现场应用提供了广阔的前景。
更新日期：2017-11-02
中文翻译：
无标签和无酶平台，具有可见输出，可使用笼状G四联体作为信号转换器来构建多功能逻辑门
使用笼状G四联体作为信号传感器，在无标记和无酶的传感平台上实现了一套完整的二进制基本逻辑门（“或”，“与非”，“与非”，“隐含”，“异或”，“异或”）。在适当的输入的存在，所述临时阻塞富含G的发夹DNA序列通过裂解由协同地稳定释放的Mg 2+ -依赖性DNA核酶，其可以使功能通过DNA核酶的输入引导的协同结合亚单位。在存在血红素的情况下，未封闭的G-四链体DNAzyme催化H 2 O 2氧化3,3'，5,5'-四甲基联苯胺（TMB）产生彩色的读出信号，该信号可以用肉眼很容易地分辨出来。对于逻辑运算，此策略非常通用且简单。还成功制造了两个组合门（XOR + AND和XOR + NOR），以演示计算元素的模块化和可扩展性。该逻辑系统的独特优势在于，水溶液中的分子事件可以转化为颜色变化，而无需借助任何分析仪器，就可以通过肉眼直接观察到颜色变化。而且，这项工作揭示了分子逻辑门设计的新途径，该方法无需任何标记和固定程序或分离和洗涤步骤即可执行，这为智能即时诊断和现场应用提供了广阔的前景。
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