Abstract Mg-Ca-TiO2 (MCT) composite scaffolds loaded with different concentrations of doxycycline (DC) with a network of interconnected pores with good compressive strength (5 ± 0.1 MPa) were fabricated via space holder method for the first time. The results showed that MCT-DC scaffolds possess a porosity and pore size in the range of 65–67% and 600–800 μm respectively. The bioactivity results exhibited the apatite formation on the MCT-DC scaffold surface, indicating that DC did not obstruct the bioactivity of MCT. The MCT-DC scaffolds drug release profiles show the initial burst and sustained drug release (55–75%) and the release rate could be adjusted via altering the DC concentration. The MCT loaded with 1 and 5% DC did not indicate cytotoxic behavior against MG63 cells while further DC loading resulted in some toxicity. Antimicrobial properties of MCT-DC scaffolds against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria were examined and the results reveal oblivious inhibition zone around each MCT-DC scaffold whereas no obvious inhibition is observed around the MCT scaffold. Therefore, MCT-DC composite scaffolds with low concentration of DC could be alternative candidates for infection prevention and bone tissue engineering.

中文翻译：

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多西环素负载Mg-Ca-TiO 2 复合支架的药物释放、细胞相容性、生物活性和抗菌活性

摘要 首次通过空间支架法制备了负载不同浓度多西环素（DC）的具有良好抗压强度（5±0.1 MPa）的互连孔隙网络的Mg-Ca-TiO2（MCT）复合支架。结果表明，MCT-DC 支架的孔隙率和孔径分别在 65-67% 和 600-800 μm 范围内。生物活性结果显示在 MCT-DC 支架表面形成磷灰石，表明 DC 没有阻碍 MCT 的生物活性。MCT-DC 支架药物释放曲线显示初始爆发和持续药物释放 (55-75%)，释放速率可以通过改变 DC 浓度来调整。加载有 1% 和 5% DC 的 MCT 没有表明对 MG63 细胞的细胞毒性行为，而进一步加载 DC 会导致一些毒性。检查了 MCT-DC 支架对金黄色葡萄球菌 (S. aureus) 和大肠杆菌 (E.coli) 细菌的抗菌特性，结果显示每个 MCT-DC 支架周围不存在抑制区，而在 MCT 支架周围没有观察到明显的抑制作用。因此，具有低浓度 DC 的 MCT-DC 复合支架可能是预防感染和骨组织工程的替代候选物。