Sardinian alcohol-preferring (sP) and Sardinian alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. Aiming to verify possible differences at proteomics level between sP and sNP rats, we investigated the salivary proteome by a a liquid chromatography-mass spectrometry top-down/bottom-up integrated approach. To this purpose submandibular saliva was collected from alcohol-naive sP and sNP rats under isoprenaline stimulation. 200 peptides/proteins were detected and quantified in the two rat lines, 149 of which were characterized in their naturally occurring structure. Data available via ProteomeXchange with identifier PXD006997. Surprisingly, sP rats exhibited marked quantitative and qualitative differences with respect to sNP rats, namely higher levels of proteoforms originating from submandibular gland protein C, and from submandibular rat protein 2, as well as of several unidentified peptides/proteins. sP rats expressed some proteins not detectable in sNP rats, as the glutamine/glutamic acid-rich protein (GRP)-CB. The isoform GRP-B, detectable in both rat lines, was more abundant in sNP rats. Submandibular saliva of sNP rats was also characterized by very high levels of GRP-B proteolytic peptides, and rat salivary protein 1. Whether these differences could contribute to the opposite alcohol preference/consumption of sP and sNP rats is currently unknown and requires further investigation.

中文翻译：

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通过整合的自上而下/自下而上的蛋白质组学平台揭示了喜好撒丁岛和不喜撒丁岛的大鼠之间的颌下唾液蛋白质组的显着差异。

撒丁岛偏爱酒精的大鼠（sP）和撒丁岛偏爱非酒精饮料（sNP）的大鼠已被选择性饲养，以提供相反的酒精偏爱和消费。为了验证sP和sNP大鼠在蛋白质组学水平上的可能差异，我们通过液相色谱-质谱联用自上而下/自下而上的综合方法研究了唾液蛋白质组。为此目的，在异丙肾上腺素刺激下，从无酒精的sP和sNP大鼠中收集下颌下唾液。在两个大鼠品系中检测并定量了200种肽/蛋白质，其中149个以其天然结构为特征。数据可通过ProteomeXchange获得，其标识符为PXD006997。令人惊讶的是，与sNP大鼠相比，sP大鼠表现出明显的数量和质量差异，即来自下颌下腺蛋白C和下颌下大鼠蛋白2以及一些未鉴定的肽/蛋白的更高水平的蛋白形。sP大鼠表达了一些在sNP大鼠中无法检测到的蛋白质，例如谷氨酰胺/富含谷氨酸的蛋白质（GRP）-CB。在两个大鼠品系中均可检测到的同工型GRP-B在sNP大鼠中更为丰富。sNP大鼠的颌下唾液还具有非常高水平的GRP-B蛋白水解肽和大鼠唾液蛋白1的特征。目前尚不清楚这些差异是否可能导致sP和sNP大鼠的相反酒精偏好/消费。作为谷氨酰胺/富含谷氨酸的蛋白质（GRP）-CB。在两个大鼠品系中均可检测到的同工型GRP-B在sNP大鼠中更为丰富。sNP大鼠的颌下唾液还具有非常高水平的GRP-B蛋白水解肽和大鼠唾液蛋白1的特征。目前尚不清楚这些差异是否可能导致sP和sNP大鼠的相反酒精偏好/消费。作为谷氨酰胺/富含谷氨酸的蛋白质（GRP）-CB。在两个大鼠品系中均可检测到的同工型GRP-B在sNP大鼠中更为丰富。sNP大鼠的颌下唾液还具有非常高水平的GRP-B蛋白水解肽和大鼠唾液蛋白1的特征。目前尚不清楚这些差异是否可能导致sP和sNP大鼠的相反酒精偏好/消费。