Solution Conformation of Polymer Brushes Determines Their Interactions with DNA and Transfection Efficiency,Biomacromolecules

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Solution Conformation of Polymer Brushes Determines Their Interactions with DNA and Transfection Efficiency
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-10-25 00:00:00 , DOI: 10.1021/acs.biomac.7b01175
Mahentha Krishnamoorthy , Danyang Li , Amir S. Sharili ₁ , Tina Gulin-Sarfraz ₂ , Jessica M. Rosenholm ₂ , Julien E. Gautrot

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Polymer brush-functionalized nanomaterials offer interesting features for the design of gene delivery vectors as their physicochemical and structural properties can be designed independently of the chemistry, size and shape of the nanomaterial core. However, little is known of the parameters regulating the adsorption and infiltration of DNA molecules at the surface of positively charged polymer brushes, despite the importance of such processes for gene delivery. Here we investigate the role of the molecular environment (e.g., pH, type of buffer, concentration) on the interactions between plasmid DNA and positively charged poly(dimethylaminoethyl methacrylate) (PDMAEMA) brushes using a combination of light scattering, electrophoretic light scattering, in situ ellipsometry, and surface plasmon resonance. We show that the conformation of swollen PDMAEMA brushes is modulated by the surrounding buffer and that this impacts strongly on the ability of such brushes and nanomaterials based on these coatings to complex DNA molecules. In turn, the levels of transfection efficiency measured correlate with changes in brush conformation and DNA binding. Therefore, this work demonstrates the importance of molecular design of polymer brushes to control DNA complexation and release in order to optimize the performance of polymer brush-functionalized nanomaterials for gene delivery applications.

中文翻译：

聚合物刷的溶液形态决定了它们与DNA的相互作用和转染效率

聚合物刷功能化的纳米材料为基因传递载体的设计提供了有趣的功能，因为其理化和结构特性可以独立于纳米材料核心的化学，尺寸和形状进行设计。然而，尽管这种方法对于基因递送很重要，但调节带正电荷的聚合物刷表面上DNA分子的吸附和渗透的参数知之甚少。在这里，我们结合使用光散射，电泳光散射，研究了分子环境（例如pH，缓冲液类型，浓度）对质粒DNA与带正电的聚甲基丙烯酸二甲基氨基乙基酯（PDMAEMA）刷子之间相互作用的作用。原位椭圆偏振法和表面等离振子共振。我们显示，溶胀的PDMAEMA刷子的构象受到周围缓冲液的调节，并且这严重影响了此类刷子和基于这些涂层的纳米材料对复杂DNA分子的纳米能力。反过来，测得的转染效率水平与刷子构象和DNA结合的变化相关。因此，这项工作证明了聚合物刷的分子设计对控制DNA络合和释放的重要性，以优化聚合物刷功能化的纳米材料在基因递送应用中的性能。所测量的转染效率水平与刷子构象和DNA结合的变化相关。因此，这项工作证明了聚合物刷的分子设计对控制DNA络合和释放的重要性，以优化聚合物刷功能化的纳米材料在基因递送应用中的性能。所测量的转染效率水平与刷子构象和DNA结合的变化相关。因此，这项工作证明了聚合物刷的分子设计对控制DNA络合和释放的重要性，以优化聚合物刷功能化的纳米材料在基因递送应用中的性能。

更新日期：2017-10-26

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