The electrochemical detection of alpha-feto protein based on novel gold nanoparticles- poly(propylene imine) dendrimer platform is reported. The platform was prepared by co-electrodeposition of gold nanoparticles and generation 3 poly (propylene imine) dendrimer on a glassy carbon electrode. Each modifying step was characterised by cyclic voltammetry and electrochemical impedance spectroscopy. The electrochemical measurements showed that the platform was stable, conducting and exhibited reversible electrochemistry. Results obtained from the electrochemical impedance spectroscopy interrogation in [Fe(CN)63−/4−] redox probe showed a marked reduction in charge transfer resistance (Rct) after each modification step. The immunosensor was prepared by immobilisation of a probe anti-alpha feto protein (AFP) on the platform for 3 hrs at 35 °C followed by blocking the surface with bovine serum albumin to minimise non-specific binding. The prepared immunosensor was used to detect AFP over a wide concentration range from 0.005 to 500 ng/mL and detection limits of 0.0022 and 0.00185 ng/mL were obtained for SWV and EIS measurements respectively. The immunosensor gave good stability over a period of fourteen days when stored at 4 °C.

中文翻译：

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用于检测甲胎蛋白 - 一种癌症生物标志物的树枝状聚合物支持的电化学免疫传感器

报道了基于新型金纳米粒子-聚（丙烯亚胺）树枝状聚合物平台的甲胎蛋白电化学检测。该平台是通过在玻碳电极上共电沉积金纳米颗粒和第 3 代聚（丙烯亚胺）树枝状聚合物制备的。每个改性步骤都通过循环伏安法和电化学阻抗谱进行表征。电化学测量表明该平台稳定、导电并表现出可逆的电化学。从 [Fe(CN)63-/4-] 氧化还原探针中的电化学阻抗谱询问获得的结果显示，在每个修饰步骤后电荷转移电阻 (Rct) 显着降低。通过将探针抗甲胎蛋白 (AFP) 在 35°C 下固定在平台上 3 小时，然后用牛血清白蛋白封闭表面以最小化非特异性结合来制备免疫传感器。制备的免疫传感器用于检测 0.005 至 500 ng/mL 的宽浓度范围内的 AFP，SWV 和 EIS 测量分别获得 0.0022 和 0.00185 ng/mL 的检测限。当在 4°C 下储存时，免疫传感器在 14 天的时间内具有良好的稳定性。