Residing at the interface of chemistry and biotechnology, artificial metalloenzymes (ArMs) offer an attractive technology to combine the versatile reaction repertoire of transition metal catalysts with the exquisite catalytic features of enzymes. While earlier efforts in this field predominantly comprised studies in well-defined test-tube environments, a trend towards exploiting ArMs in more complex environments has recently emerged. Integration of these artificial biocatalysts in enzymatic cascades and using them in whole-cell biotransformations and in vivo opens up entirely novel prospects for both preparative chemistry and synthetic biology. We highlight selected recent developments with a particular focus on challenges and opportunities in the in vivo application of ArMs.

人工金属酶（ArMs）处于化学和生物技术的交界处，它提供了一种有吸引力的技术，可以将过渡金属催化剂的多种反应方式与酶的精妙催化特性相结合。尽管该领域的早期工作主要包括在定义明确的试管环境中进行研究，但最近出现了在更复杂的环境中利用ArM的趋势。将这些人工生物催化剂整合到酶促级联反应中，并将其用于全细胞生物转化和体内，为制备化学和合成生物学开辟了全新的前景。我们重点介绍了最近的一些发展，特别着重于ArM体内应用中的挑战和机遇。