This work was conducted in order to evaluate an instance of bioaugmentation, namely, the addition of a novel p-nitrophenol (PNP)-degrading bacterium Methylobacterium sp. C1 coaggregated with two other broad-spectrum coaggregating strains (Bacillus megaterium T1 and Bacillus cereus G5) within sequence batch biofilm reactors (SBBRs). Results showed that biofilms consisting of C1 and coaggregating bacteria were resistant to shock loads and were more efficient at PNP removal. High-throughput sequencing data revealed that biofilms formed in the presence of the coaggregating bacteria demonstrated greater microbial diversity. These results suggest that broad-spectrum coaggregating bacteria may be capable of mediating the immobilization of exogenous degrading bacteria into biofilms, rendering them more resistant to toxic compounds and environmental stresses. This represents the first attempt to assess the bioaugmentation of PNP-contaminated wastewater treatment through the utilization of broad-spectrum coaggregating bacteria.

中文翻译：

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菌株Methylobacterium sp。的生物增强。批量批处理生物膜反应器中具有广谱聚集细菌的C1对对硝基苯酚的去除

进行这项工作是为了评估生物增强的实例，即添加新的对硝基苯酚（PNP）降解细菌Methylobacterium sp.。C1与其他两个广谱共菌株（巨芽孢杆菌T1和蜡状芽孢杆菌）共聚集G5）在顺序分批生物膜反应器（SBBR）中。结果表明，由C1和共聚集细菌组成的生物膜对冲击负荷具有抵抗力，并且在去除PNP方面更有效。高通量测序数据显示，在存在共聚集细菌的情况下形成的生物膜表现出更大的微生物多样性。这些结果表明，广谱聚集细菌可能能够将外源降解细菌固定化为生物膜，从而使其对有毒化合物和环境压力更具抵抗力。这是通过利用广谱共聚细菌评估PNP污染的废水处理的生物强化的首次尝试。