The cyanine dye (1) with three arms of one chromenyl group and two indol groups was synthesized in this paper; and the proposed synthetic mechanism was also mentioned. It has excellent photostability compared to three typical cyanine dyes. Dye 1 gives high chemical activity and nearly turn-on fluorescent response towards hydrosulfide at 600–800 nm, it also has good selectivity towards hydrosulfide over other species; the detection reaction mechanism was proved by ¹H NMR analysis, and the emission responses was elucidated by time dependent density functional theory (TDDFT) calculation. The in cellular fluorescent imaging application of the dye 1 was successfully performed for the hydrosulfide imaging of HeLa cells.

合成了具有一个色烯基和两个吲哚基的三臂的花青染料（1）；还提到了拟议的综合机制。与三种典型的花青染料相比，它具有出色的光稳定性。染料1具有很高的化学活性，并且在600–800 nm时对氢硫化物具有几乎开启的荧光响应，它对氢硫化物的选择性也比其他物种好；通过¹ H NMR分析证明了检测反应的机理，并通过时变密度泛函理论（TDDFT）计算阐明了发射反应。染料1在细胞内荧光成像中的应用已成功地用于HeLa细胞的氢硫化物成像。