The measurement of Na⁺, K⁺ and H⁺ is essential in medicine and plays an important role in the assessment of tissue ischemia. Microfabrication, inkjet- and screen-printing can be used for solid contact ion selective electrodes (ISE) realization; these, however, can be non-standardized, costly and time consuming processes. We present the realization of ISEs on post-processed electrodes fabricated via standardized printed circuit board (PCB) manufacturing techniques. In vitro results are presented from two rigid platforms (32 ISEs) for liquid sample dip-stick measurements and two flexible platforms (6 and 32 ISEs) for post-surgical intestinal tissue monitoring, each with a common reference electrode (RE). These are combined with optimized tetrapolar bioimpedance sensors for tissue ischemia detection. Both electroless and hard gold PCB finishes are examined. Apart from the electroless rigid platform, the rest demonstrated comparable and superior performance, with the pH sensors demonstrating the greatest deviation; the flexible hard gold platform achieved a sensitivity 4.6 mV/pH and 49.2 mV/pH greater than the electroless flexible and rigid platforms, respectively. The best overall performance was achieved with the hard gold flexible platform with sensitivities as large as 73.4 mV/pH, 56.3 mV/log [Na⁺], and 57.4 mV/log [K⁺] vs. custom REs on the same substrate. Simultaneous measurement of target analytes is demonstrated with test solutions and saliva samples. The results demonstrate superior performance to other PCB-based pH sensors and Na⁺ and K⁺ PCB-based sensors with comparable performance to potentiometric sensors fabricated with other techniques, paving the way towards mass-produced, low-cost, disposable, multi-parametric chemical sensing diagnostic platforms.

Na ⁺ ， K ⁺和H ⁺的测量在医学中是必不可少的，并且在组织缺血性评估中起着重要作用。可以将微细加工，喷墨印刷和丝网印刷用于实现固体接触离子选择电极（ISE）；但是，这些过程可能是非标准化，昂贵且耗时的过程。我们介绍了通过标准印刷电路板（PCB）制造技术制造的后处理电极上的ISE实现。体外结果来自用于液体样品浸量测量的两个刚性平台（32 ISE）和用于术后肠组织监测的两个柔性平台（6和32 ISE），每个平台都有一个公共参比电极（RE）。这些与优化的四极生物阻抗传感器结合在一起用于组织缺血检测。对化学镀金​​和硬金PCB镀层均进行了检查。除了无电的刚性平台以外，其余的均表现出可比的和优越的性能，其中pH传感器显示出最大的偏差。柔性硬金平台的灵敏度分别比化学柔性和刚性平台高4.6 mV / pH和49.2 mV / pH。使用具有高达73.4 mV / pH，56.3 mV / log [Na [Na]⁺ ]和57.4 mV / log [K ⁺ ]对比同一基板上的自定义RE。通过测试溶液和唾液样品可以同时测量目标分析物。结果表明，其性能优于其他基于PCB的pH传感器以及基于Na ⁺和K ⁺ PCB的传感器，其性能可与采用其他技术制造的电位传感器相媲美，从而为批量生产，低成本，一次性，多参数化铺平了道路。化学传感诊断平台。