A sandwich-configuration electrochemiluminescence (ECL) immunosensor based on Au [email protected] nanocomposites for ultrasensitive determination of human chorionic gonadotropin (HCG) has been developed. In this work, nanostructured Polyaniline hydrogels (Pani) decorated with Pt nanoparticles (Pani/Pt) were utilized to construct the base of this immunosensor, greatly increasing the amount of loaded capture antibodies (Ab₁) via linkage reagent glutaraldehyde (GA). The used conducting Pani/Pt nanocomposites possessed the unique features, such as large surface area, high electron transfer speed and favorable electrocatalytic activities of hydrogen peroxide, which offered a prominent platform for this sandwich-sensor and acted as efficient ECL signal amplifier also. Furthermore, we employed horseradish peroxidase (HRP) to block the nonspecific binding sites instead of commonly used bovine serum albumin (BSA), which further amplified the signal of luminol in the present of hydrogen peroxide (H₂O₂). In addition, Au [email protected] nanocomposites with excellent water solubility, low-toxicity and great biocompatibility were prepared and used to immobilize HCG detection antibodies (Ab₂) and luminescent material luminol. Then, the above-synthetized Luminol-Au [email protected]₂ complex was attached to the modified sensor by sandwiched immunoreactions. Under the optimized conditions, the proposed immunosensor exhibited a sensitive detection of HCG in a wide linear range from 0.001 to 500 mIU mL⁻¹ with a detection limit of 0.0003 mIU/mL (S/N = 3). All the results indicated that such a sandwiched HCG immunosensor exhibited favorable ECL analytical performance. This developed method may be potentially used to recognize other clinical protein and display a novel ideal to construct an immunosensor.

已开发出一种基于Au [电子邮件保护]纳米复合材料的三明治构型电化学发光（ECL）免疫传感器，用于超灵敏测定人绒毛膜促性腺激素（HCG）。在这项工作中，利用装饰有Pt纳米颗粒（Pani / Pt）的纳米结构聚苯胺水凝胶（Pani）来构建此免疫传感器的基础，从而大大增加了负载捕获抗体的量（Ab ₁）通过连接试剂戊二醛（GA）。所用的导电Pani / Pt纳米复合材料具有比表面积大，电子转移速度快，过氧化氢具有良好的电催化活性等独特特征，为该夹心传感器提供了重要的平台，还可以作为高效的ECL信号放大器。此外，我们使用辣根过氧化物酶（HRP）来阻断非特异性结合位点，而不是通常使用的牛血清白蛋白（BSA），后者在存在过氧化氢（H ₂ O ₂）的情况下进一步放大了鲁米诺的信号。此外，制备了具有优异水溶性，低毒性和良好生物相容性的金[受电子邮件保护的]纳米复合材料，并将其用于固定HCG检测抗体（Ab ₂）和发光材料luminol。然后，将上述合成的Luminol-Au [电子邮件保护的] ₂复合物通过夹心免疫反应连接到修饰的传感器上。在最佳条件下，拟议的免疫传感器在0.001至500 mIU mL ^-1的宽线性范围内显示出对HCG的灵敏检测，检测极限为0.0003 mIU / mL（S / N = 3）。所有结果表明，这种夹心HCG免疫传感器表现出良好的ECL分析性能。这种开发的方法可能潜在地用于识别其他临床蛋白质并显示出构建免疫传感器的新理想。