A novel impedimetric biosensor was developed for the detection of the two extensively used pesticides, acetamiprid and atrazine. By employing the sputtering and e-beam lithography techniques, platinum nanoparticles (Pt NPs) were deposited in a bridge-like arrangement, in between interdigitated electrodes (IDEs). The resulting Pt NP microwires were chemically functionalized to allow the covalent immobilization of aptamers against the two target analytes onto the sensor surfaces. The biosensing platform facilitated charge transfer through the microwire-bridged IDEs, while upon analyte binding to the immobilized aptamers electron transfer was hindered, resulting in an increase of the electrochemical cell's impedance. The combination of Pt NPs microwires and aptamers allowed the sensitive and highly selective detection of acetamiprid with a linear range of response in the range of 10 pM to 100 nM with a limit of detection (LoD) at 1 pM, and of atrazine with a linear range of responses from 100 pM to 1 μM and a LoD at 10 pM respectively. Its performance was tested against a number of other commonly used pesticides as well as in real water samples.

开发了一种新型的阻抗生物传感器，用于检测两种广泛使用的杀虫剂扑热息痛和at去津。通过采用溅射和电子束光刻技术，铂纳米颗粒（Pt NPs）以桥状排列沉积在叉指电极（IDE）之间。对所得的Pt NP微丝进行化学功能化，以将适体针对两种目标分析物的共价固定在传感器表面上。生物传感平台促进了电荷通过微丝桥IDE的转移，而当分析物与固定适体结合时，电子转移受到阻碍，从而导致电化学电池阻抗的增加。Pt NPs微丝和适体的组合允许灵敏且高度选择性地检测扑热息痛，其线性响应范围为10 pM至100 nM，检测限（LoD）为1 pM，而at去津具有线性响应范围从100 pM到1μM，LoD分别为10 pM。该产品的性能已针对多种其他常用农药以及真实水样进行了测试。