Electrophoretic Deposition of Gentamicin-Loaded Silk Fibroin Coatings on 3D-Printed Porous Cobalt–Chromium–Molybdenum Bone Substitutes to Prevent Orthopedic Implant Infections,Biomacromolecules

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Electrophoretic Deposition of Gentamicin-Loaded Silk Fibroin Coatings on 3D-Printed Porous Cobalt–Chromium–Molybdenum Bone Substitutes to Prevent Orthopedic Implant Infections
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-10-16 00:00:00 , DOI: 10.1021/acs.biomac.7b01091
Changjun Han ₁ , Yao Yao ₂ , Xian Cheng _{2,

3} , Jiaxin Luo ₂ , Pu Luo ₂ , Qian Wang ₁ , Fang Yang ₃ , Qingsong Wei ₁ , Zhen Zhang ₂

Affiliation

In addition to customizing shapes of metal bone substitutes for patients, the 3D printing technique can reduce the modulus of the substitutes through the design and manufacture of interconnected porous structures, achieving the modulus match between substitute and surrounding bone to improve implant longevity. However, the porous bone substitutes take more risks of postoperative infection due to its much larger surface area compared with the traditional casting solid bone substitute. Here, we prepared of gentamicin-loaded silk fibroin coatings on 3D-printed porous cobalt–chromium–molybdenum (CoCrMo) bone substitutes via electrophoretic deposition technique. Through optimization, relatively intact, continuous, homogeneous, and conformal coatings with a thickness of 2.30 ± 0.58 μm were deposited around the struts with few pore blocked. The porous metal structures exhibited no loss in mechanical properties after the anode galvanic corrosion in EPD process. The initial osteoblastic response on coatings was better than that on metal surface, including cell spreading, proliferation and cytotoxicity. Antibacterial efficacy experiments showed that the coatings had an antibacterial effect on both adherent and planktonic bacteria within 1 week. These results suggested that the beneficial properties of anode electrophoretic deposited silk fibroin coatings could be exploited to improve the biological functionality of porous structures made of medical metals.

中文翻译：

庆大霉素负载的丝素蛋白涂层在3D打印的多孔钴-铬-钼骨替代物上的电泳沉积，以防止骨科植入物感染

除了为患者定制金属骨替代物的形状外，3D打印技术还可以通过设计和制造互连的多孔结构来降低替代物的模量，从而实现替代物与周围骨骼之间的模量匹配，从而提高植入物的寿命。但是，与传统的铸造实心骨替代物相比，多孔骨替代物具有更大的表面积，因此具有更大的术后感染风险。在这里，我们通过电泳沉积技术在3D打印的多孔钴-铬-钼（CoCrMo）骨替代物上制备了载有庆大霉素的丝素蛋白涂层。通过优化，将相对完整，连续，均匀和共形的涂层（厚度为2.30±0.58μm）沉积在支撑杆周围，几乎没有孔被堵塞。在EPD工艺中阳极电蚀后，多孔金属结构没有表现出机械性能的损失。涂层的初始成骨反应优于金属表面，包括细胞扩散，增殖和细胞毒性。抗菌功效实验表明，该涂料在1周内对附着细菌和浮游细菌均具有抗菌作用。这些结果表明，可以利用阳极电泳沉积的丝素蛋白涂层的有益性能来改善由医用金属制成的多孔结构的生物功能。增殖和细胞毒性。抗菌功效实验表明，该涂料在1周内对附着细菌和浮游细菌均具有抗菌作用。这些结果表明，可以利用阳极电泳沉积的丝素蛋白涂层的有益性能来改善由医用金属制成的多孔结构的生物功能。增殖和细胞毒性。抗菌功效实验表明，该涂料在1周内对附着细菌和浮游细菌均具有抗菌作用。这些结果表明，可以利用阳极电泳沉积的丝素蛋白涂层的有益性能来改善由医用金属制成的多孔结构的生物功能。

更新日期：2017-10-16

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