A great body of evidence unveiled the existence of different stem cell types in various tissues with efficient capability for self-renewal and differentiation. Characterization and enumeration of a specific stem cell population seem difficult because these cells consist of a small percentage of cells within different milieu. As such, due to lack of accuracy provided by convenient methods, biosensing techniques are at the center of attention to improve stem cells characterization with high rate of sensitivity and specificity. In line with this statement, bio-sensing, as a sensitive analytical method, opens novel avenues in biological aspects. Physicochemical signals produced by the interaction of analyte-ligands could be applicable for a wide range of scientific fields such as chemical, biochemical, pharmaceutical and immunological purposes. Noninvasive and label free monitoring methods without need to stain, fix, lyse, or fluorescent-tags are desirable in basic stem cell technology and therapeutic applications of cells. We, here, highlighted basic concepts and terms associated with label free biosensor technology for measuring cell entity, evaluating cell surface marker, and peculiarly in the field of stem cell biology. Furthermore, different aspects and methodologies concerning with bio-sensing techniques, including photonic-based detection systems like Surface Plasmon Resonance (SPR) assays, Impedance-based method, Quartz Crystal Microbalance (QCM) and paper based detection of lateral flow biosensor (LFB) were discussed.

大量证据揭示了具有有效自我更新和分化能力的各种组织中存在不同干细胞类型。特定干细胞群的表征和计数似乎很困难，因为这些细胞由不同环境中的一小部分细胞组成。因此，由于便利方法所缺乏的准确性，生物传感技术成为人们关注的焦点，以高灵敏度和特异性的速率改善干细胞的表征。根据这一说法，生物传感作为一种敏感的分析方法，在生物学方面开辟了新的途径。由分析物-配体相互作用产生的物理化学信号可能适用于广泛的科学领域，例如化学，生物化学，药物和免疫学目的。在基本干细胞技术和细胞治疗应用中，需要不需要染色，固定，裂解或荧光标签的无创且无标签的监测方法。在这里，我们重点介绍了与无标记生物传感器技术相关的基本概念和术语，这些技术用于测量细胞实体，评估细胞表面标志物，并且在干细胞生物学领域中尤为如此。此外，与生物传感技术有关的不同方面和方法，包括基于光子的检测系统，例如表面等离子体共振（SPR）分析，基于阻抗的方法 着重介绍了与无标记生物传感器技术相关的基本概念和术语，这些技术用于测量细胞实体，评估细胞表面标志物，特别是在干细胞生物学领域。此外，与生物传感技术有关的不同方面和方法，包括基于光子的检测系统，例如表面等离子体共振（SPR）分析，基于阻抗的方法 着重介绍了与无标记生物传感器技术相关的基本概念和术语，这些技术用于测量细胞实体，评估细胞表面标志物，特别是在干细胞生物学领域。此外，与生物传感技术有关的不同方面和方法，包括基于光子的检测系统，例如表面等离子体共振（SPR）分析，基于阻抗的方法讨论了石英微天平（QCM）和基于纸的横向流生物传感器（LFB）检测。