A Flexible Polyphosphate‐Driven Regeneration System for Coenzyme A Dependent Catalysis,ChemCatChem

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A Flexible Polyphosphate‐Driven Regeneration System for Coenzyme A Dependent Catalysis
ChemCatChem ( IF 3.8 ) Pub Date : 2017-10-16 , DOI: 10.1002/cctc.201700848
Silja Mordhorst ₁ , Alice Maurer ₁ , Désirée Popadić ₁ , Johanna Brech ₁ , Jennifer N. Andexer ₁

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Coenzyme A (CoA) is a common cofactor in biochemical reactions, and CoA‐dependent enzymes catalyze essential steps in anabolism and catabolism. This complex molecule also plays an important role in the synthesis of many high‐value products, such as synthetic antibiotics, vitamins, pheromones, and biopolymers. Nevertheless, the synthetic potential for biocatalytic processes cannot be fully exploited owing to the lack of an efficient regeneration system. Here, we report an acyl‐CoA regeneration system with integrated adenosine triphosphate (ATP) regeneration that is based on inexpensive polyphosphate as the single energy source. In the four‐enzyme cascade, two cofactors, acyl‐CoA and ATP, are each regenerated up to 2000 times. The applicability for different acyl donors and acceptors is shown by HPLC analysis. Owing to its flexibility toward virtually all relevant substrates, the system has the potential to make CoA‐dependent reactions more accessible for chemical synthesis in vitro.

中文翻译：

用于辅酶A依赖催化的灵活的多磷酸盐驱动再生系统

辅酶A（CoA）是生化反应中常见的辅因子，依赖于CoA的酶催化合成代谢和分解代谢中的关键步骤。这种复杂的分子在许多高价值产品的合成中也起着重要作用，例如合成抗生素，维生素，信息素和生物聚合物。然而，由于缺乏有效的再生系统，生物催化过程的合成潜力无法得到充分利用。在这里，我们报告了一种基于酰基磷酸三乙腺苷（ATP）再生的酰基辅酶A再生系统，该再生系统基于廉价的多磷酸盐作为单一能源。在四酶级联反应中，两个辅因子酰基辅酶A和ATP分别再生多达2000次。通过HPLC分析显示了对于不同的酰基供体和受体的适用性。

更新日期：2017-10-16

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