Direct detection of targets in complex biological media with conventional biosensors is an enormous challenge due to the nonspecific adsorption and severe biofouling. In this work, a facile strategy for sensitive and low fouling detection of adenosine triphosphate (ATP) is developed through the construction of a mixed self-assembled biosensing interface, which was composed of zwitterionic peptide (antifouling material) and ATP aptamer (bio-recognition element). The peptide and aptamer (both containing thiol groups) were simultaneously self-assembled onto gold electrode surface electrodeposited with gold nanoparticles. The developed aptasensor possessed high selectivity and sensitivity for ATP, and it showed a wide linear response range towards ATP from 0.1 pM to 5 nM. Owing to the presence of peptide with excellent antifouling property in the biosensing interface, the aptasensor can detect ATP in complex biological media with remarkably reduced biofouling or nonspecific adsorption effect. Moreover, it can directly detect ATP in 1% human whole blood without suffering from any significant interference, indicating its great potential for practical assaying of ATP in biological samples.

由于非特异性吸附和严重的生物污染，使用常规生物传感器直接检测复杂生物介质中的靶标是一个巨大的挑战。在这项工作中，通过构建由两性离子肽（抗污物质）和ATP适体（生物识别）组成的混合自组装生物传感界面，开发了一种灵敏且低污染的三磷酸腺苷（ATP）检测的简便策略。元素）。同时将肽和适体（均含有硫醇基）自组装到电沉积有金纳米颗粒的金电极表面上。研制的适体传感器对ATP具有很高的选择性和敏感性，对ATP的线性响应范围很广，从0.1 pM到5 nM。由于在生物传感界面中存在具有优异防污性能的肽，aptasensor可以在复杂的生物介质中检测到ATP，而生物污损或非特异性吸附效果显着降低。此外，它可以直接检测1％的人类全血中的ATP，而不会受到任何明显的干扰，这表明它具有实际测定生物样品中ATP的巨大潜力。