Methanol is a poison which is frequently discovered in alcoholic beverages. Innovative methods to detect methanol in alcoholic beverages are being constantly developed. We report for the first time a new strategy for the detection of methanol using fluorescence spectroscopy and photoelectrochemical (PEC) analysis. The analytical system is based on the oxidation of cysteine (CSH) with hydrogen peroxide (H₂O₂) enzymatically generated by alcohol oxidase (AOx). H₂O₂ oxidizes capping agent CSH, modulating the growth of CSH-stabilized cadmium sulphide quantum dots (CdS QDs). Disposable screen-printed carbon electrodes (SPCEs) modified with a conductive osmium polymer (Os-PVP) complex were employed to quantify resulting CdS QDs. This polymer facilitates the “wiring” of in situ enzymatically generated CdS QDs, which photocatalyze oxidation of 1-thioglycerol (TG), generating photocurrent as the readout signal. Likewise, we proved that our systems did not suffer from interference by ethanol. The PEC assays showed better sensitivity than conventional methods, covering a wide range of potential applications for methanol quantification.

甲醇是一种有毒物质，经常在酒精饮料中发现。检测酒精饮料中甲醇的创新方法正在不断发展。我们首次报告了一种使用荧光光谱和光电化学（PEC）分析检测甲醇的新策略。该分析系统基于半胱氨​​酸（CSH）被乙醇氧化酶（AOx）酶促生成的过氧化氢（H ₂ O ₂）氧化。H ₂ O ₂氧化封端剂CSH，调节CSH稳定的硫化镉量子点（CdS QDs）的生长。用导电聚合物（Os-PVP）复合物修饰的一次性丝网印刷碳电极（SPCE）用于量化生成的CdS QD。这种聚合物促进了原位酶促生成的CdS QD的“接线”，该CdS QD光催化1-硫代甘油（TG）的氧化，产生光电流作为读出信号。同样，我们证明了我们的系统不受乙醇干扰。PEC分析显示出比传统方法更好的灵敏度，涵盖了甲醇定量的广泛潜在应用。