AbstractA versatile and cost-effective aptamer-based fluorescence quenching assay is described for the detection of the mycotoxin zearalenone (ZEN). Exfoliated functional graphene oxide (FGO) of high water-dispersibility is adopted as an effective fluorescence quencher of the fluorescence of FAM. Quenching properties of graphite, graphene oxide (GO) and FGO were investigated, and FGO is found to be the most efficient quencher. FGO therefore was used in an aptamer-based detection format that allows ZEN to be determined in the concentration range of 0.5 to 64 ng·mL−1 with a limit of detection of 0.5 ng·mL−1. The aptamer assay has good repeatability and reproducibility (n ≥ 4). Selectivity of the aptamer assay against a set of possible interferents is substantiated. This aptasensing assay was successfully applied to the determination of ZEN in (spiked) alcoholic beverage samples, beer and wine, and recovery values in the range of 87 to 96% were obtained for the determination of ZEN at levels as low as 1–16 ng mL−1. Graphical abstractComparison of fluorescence quenching efficiency of graphite, graphene oxide (GO) and functional graphene oxide (FGO). Fluorophore: Fluorescein amidite (FAM).

中文翻译：

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基于使用荧光素标记和功能性氧化石墨烯作为猝灭剂的基于适体的玉米赤霉烯酮测定

摘要描述了一种用于检测霉菌毒素玉米赤霉烯酮 (ZEN) 的通用且经济高效的基于适配体的荧光猝灭试验。采用高水分散性的剥离功能氧化石墨烯 (FGO) 作为 FAM 荧光的有效荧光猝灭剂。研究了石墨、氧化石墨烯 (GO) 和 FGO 的淬火性能，发现 FGO 是最有效的淬火剂。因此，FGO 用于基于适配体的检测形式，允许在 0.5 至 64 ng·mL-1 的浓度范围内测定 ZEN，检测限为 0.5 ng·mL-1。适配体检测具有良好的重复性和再现性（n≥4）。证实了适体测定对一组可能干扰物的选择性。该适敏检测成功应用于（加标）酒精饮料样品、啤酒和葡萄酒中 ZEN 的测定，并且获得了 87% 至 96% 范围内的回收率值，可用于测定低至 1-16 ng 的 ZEN毫升-1。石墨、氧化石墨烯 (GO) 和功能性氧化石墨烯 (FGO) 的荧光猝灭效率的图形摘要比较。荧光团：荧光素酰胺（FAM）。