AbstractThe authors describe an oligonucleotide-based lateral flow test for visual detection of Ag(I). The assay is based on cytosine-Ag(I)-cytosine [C-Ag(I)-C] coordination chemistry to capture gold nanoparticle (AuNP) tags in the test zone. A thiolated C-rich oligonucleotide probe was immobilized on the AuNPs via gold-thiol chemistry, and a biotinylated C-rich oligonucleotide probe was immobilized on the test zone. The AuNPs labelled with C-rich oligonucleotides are captured by Ag(I) ions in the test zone through the C-Ag(I)-C coordination. The resulting accumulation of AuNPs produces a readily visible red band in the test zone. Under optimized conditions, the test is capable of visually detecting 1.0 ppb of Ag(I) which is 50 times lower than the maximum allowable concentration as defined by the US Environmental Protection Agency for drinking water. Hence, the test is inexpensive and highly sensitive. It was applied to the detection of Ag(I) in spiked samples of tap water and river water. In our perception, the test is a particularly valuable tool in limited resource settings. Graphical abstractGraphical Abstract

中文翻译：

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基于富含 C 寡核苷酸中胞嘧啶-Ag(I)-胞嘧啶相互作用的侧流层析测试，用于视觉检测银(I)


摘要作者描述了一种基于寡核苷酸的侧向层析测试，用于视觉检测 Ag(I)。该测定基于胞嘧啶-Ag(I)-胞嘧啶 [C-Ag(I)-C] 配位化学，以捕获测试区中的金纳米颗粒 (AuNP) 标签。通过金硫醇化学将硫醇化的富含 C 的寡核苷酸探针固定在 AuNP 上，并将生物素化的富含 C 的寡核苷酸探针固定在测试区上。测试区中的 Ag(I) 离子通过 C-Ag(I)-C 配位捕获用富含 C 的寡核苷酸标记的 AuNP。由此产生的金纳米颗粒积累会在测试区域产生清晰可见的红色条带。在优化条件下，该测试能够目视检测 1.0 ppb 的 Ag(I)，比美国环境保护局规定的饮用水最大允许浓度低 50 倍。因此，该测试成本低廉且高度敏感。应用于自来水和河水中加标样品中Ag(I)的检测。在我们看来，在有限的资源环境中，测试是一个特别有价值的工具。 Graphical AbstractGraphic Abstract图形摘要