AbstractThe authors describe a method for signal amplification of label-free voltammetric immunosensors. A glassy carbon electrode (GCE) was modified with Prussian Blue-platinum nanoparticles (PB-PtNPs) as a redox-active species that gives a strong amperometric signal at 0.18 V (vs. Ag/AgCl). Benefitting from the excellent electrical conductivity and the strong catalytic activity to H2O2, the modified GCE gives a strongly enhanced signal. The PB-PtNPs were incorporated into a polyaniline (PANI) hydrogel to further enhance the signal. The signal response of the PB-PtNP-PANI/GCE is larger by a factor of 7.6 than that of PB-PtNP/GCE. In order to further improve electrical conductivity and immobilize antibody, gold nanoparticles (AuNPs) were deposited on the surface of the PB-PtNP-PANI hydrogel. The AuNP-PB-PtNP-PANI hydrogel nanocomposite on the GCE was used in an immunosensor for the model analyte carcinoma antigen 125 (CA125), a biomarker for epithelial ovarian cancer, by immobilizing the respective antibody on the modified GCE. A linear response found for the 0.01 to 5000 U mL−1 CA125 concentration range, with a detection limit of 4.4 mU mL−1 (at an S/N ratio of 3). The electrochemical sensitivity is as high as 119.76 μA·(U/mL)−1·cm−2. The detection of CA125 in human serum showed satisfactory accuracy compared to a commercial chemiluminescent microparticle immunoassay (CMIA). Graphical abstractSchematic of a nanocomposites consisting of gold nanoparticles, Prussian Blue, platinum nanoparticles and polyaniline hydrogel as a signal multi-amplification sensing substrate for the ultrasensitive immuno detection of carcinoma antigen 125 (CA125).

中文翻译：

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一种含有普鲁士蓝、铂纳米颗粒和聚苯胺的纳米复合材料，用于伏安免疫传感器信号的多重放大：癌抗原 125 的高灵敏度检测

摘要作者描述了一种无标记伏安免疫传感器的信号放大方法。玻碳电极 (GCE) 用普鲁士蓝铂纳米粒子 (PB-PtNPs) 作为氧化还原活性物质进行了修饰，在 0.18 V（相对于 Ag/AgCl）时提供了强电流信号。得益于优异的导电性和对 H2O2 的强催化活性，改性 GCE 提​​供了强烈增强的信号。PB-PtNPs 被掺入聚苯胺 (PANI) 水凝胶中以进一步增强信号。PB-PtNP-PANI/GCE 的信号响应比 PB-PtNP/GCE 大 7.6 倍。为了进一步提高电导率和固定抗体，金纳米粒子 (AuNPs) 沉积在 PB-PtNP-PANI 水凝胶的表面。GCE 上的 AuNP-PB-PtNP-PANI 水凝胶纳米复合材料用于模型分析物癌抗原 125（CA125）的免疫传感器，这是一种上皮性卵巢癌的生物标志物，通过将相应的抗体固定在修饰的 GCE 上。在 0.01 至 5000 U mL-1 CA125 浓度范围内发现线性响应，检测限为 4.4 mU mL-1（信噪比为 3）。电化学灵敏度高达119.76 μA·(U/mL)-1·cm-2。与商业化学发光微粒免疫分析 (CMIA) 相比，人血清中 CA125 的检测显示出令人满意的准确性。由金纳米颗粒、普鲁士蓝、