A novel electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of carcinoembryonic antigen (CEA) was developed using signal amplification strategy based on poly(5-formylindole)/reduced graphene oxide nanocomposite (P5FIn/erGO) and Au nanoparticle (AuNP) decorated graphene quantum dots (GQDs) ([email protected]). As an effective matrix for immobilization of primary antibody (Ab1), P5FIn/erGO nanocomposite facilitated the ion transport during the redox reactions and provided larger surface areas for the immobilization of Ab1. [email protected] was used as labels to conjugate with secondary antibody (Ab2), which improved electron transfer capability with stable ECL intensity. The multiple amplification of P5FIn/erGO and [email protected] made the ECL immunosensor have a broad linear range from 0.1 pg mL⁻¹ to 10 ng mL⁻¹ and a low detection limit with 3.78 fg mL⁻¹. In addition, this ECL immunosensor performed with admirable stability and good selectivity and reproducibility as well. When this immunosensor was used for the analysis of CEA in human serum, good recoveries were obtained. Thus, there will be a promising future in the early diagnosis of cancer to detect CEA.

基于聚（5-甲酰基吲哚）/还原石墨烯氧化物纳米复合材料（P5FIn / erGO）和金纳米颗粒（AuNP）修饰的石墨烯量子点的信号放大策略，开发了一种用于超灵敏检测癌胚抗原（CEA）的新型电化学发光（ECL）免疫传感器。 （GQD）（[受电子邮件保护]）。作为固定一抗（Ab1）的有效基质，P5FIn / erGO纳米复合材料促进了氧化还原反应过程中的离子迁移，并为固定Ab1提供了更大的表面积。[电子邮件保护]用作与第二抗体（Ab2）缀合的标记，从而以稳定的ECL强度提高了电子转移能力。P5FIn / erGO的多重扩增和[电子邮件保护]使ECL免疫传感器具有从0.1 pg mL ^-1的宽线性范围至10 ng mL ^-1和低检测限3.78 fg mL ^-1。此外，该ECL免疫传感器的性能也令人赞叹，稳定性，选择性和重现性也很好。当使用该免疫传感器分析人血清中的CEA时，获得了良好的回收率。因此，在早期诊断癌症以检测CEA方面将有希望的未来。