The spliceosome excises introns from pre-messenger RNAs using an RNA-based active site that is cradled by a dynamic protein scaffold. A recent revolution in cryo-electron microscopy (cryo-EM) has led to near-atomic-resolution structures of key spliceosome complexes that provide insight into the mechanism of activation, splice site positioning, catalysis, protein rearrangements and ATPase-mediated dynamics of the active site. The cryo-EM structures rationalize decades of observations from genetic and biochemical studies and provide a molecular framework for future functional studies.

中文翻译：

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剪接体的低温电子显微镜快照：动态核糖核蛋白机器的结构见解

剪接体使用由动态蛋白支架支撑的基于RNA的活性位点从信使前RNA中切除内含子。低温电子显微镜（cryo-EM）的最新革命已导致关键剪接体复合物的接近原子分辨率的结构，从而提供了对激活机制，剪接位点定位，催化，蛋白质重排和ATPase介导的PPAR动力学的深入了解。活动站点。cryo-EM结构合理化了数十年来遗传和生化研究的观察结果，并为未来的功能研究提供了分子框架。