The spliceosome excises introns from pre-messenger RNAs using an RNA-based active site that is cradled by a dynamic protein scaffold. A recent revolution in cryo-electron microscopy (cryo-EM) has led to near-atomic-resolution structures of key spliceosome complexes that provide insight into the mechanism of activation, splice site positioning, catalysis, protein rearrangements and ATPase-mediated dynamics of the active site. The cryo-EM structures rationalize decades of observations from genetic and biochemical studies and provide a molecular framework for future functional studies.

中文翻译：

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剪接体的冷冻电子显微镜快照：动态核糖核蛋白机器的结构洞察

剪接体使用由动态蛋白质支架支撑的基于 RNA 的活性位点从前信使 RNA 中切除内含子。最近低温电子显微镜 (cryo-EM) 的革命导致关键剪接体复合物的近原子分辨率结构提供了对激活机制、剪接位点定位、催化、蛋白质重排和 ATP 酶介导的动力学的深入了解。活跃的网站。冷冻电镜结构使几十年来遗传和生化研究的观察结果合理化，并为未来的功能研究提供了分子框架。