Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples,Electroanalysis

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Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples
Electroanalysis ( IF 2.7 ) Pub Date : 2017-09-25 , DOI: 10.1002/elan.201700428
Hamid R. Lotfi Zadeh Zhad ₁ , Rebecca Y. Lai ₁

Affiliation

We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration-dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration-dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 % synthetic urine and 25 % synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.

中文翻译：

铁（III）介导的复杂生物样品中左氧氟沙星的电化学检测

我们报告了一种能够检测复杂生物样品中左氧氟沙星 (LEVX) 的电化学传感器的设计。这种检测策略简单、快速，不需要样品预处理或电极修改。与之前开发的需要 LEVX 直接氧化的电化学 LEVX 传感器不同，传感机制基于 LEVX 和铁 (III) 之间的络合反应，导致铁 (III) 还原峰值电流的浓度依赖性降低和峰值电位。这些变化可能归因于溶液中未络合的 Fe(III) 浓度的降低。电流和电位的浓度依赖性变化可用于量化各种样品中的 LEVX，包括 50% 合成尿液和 25% 合成人唾液。检测限估计在 1.5 到 2.3 μM 的范围内，浓度远低于在尿路感染等病症中使用该药物的患者的尿液和唾液样本中发现的 LEVX 浓度。通过进一步优化，这种传感策略可以在临床药代动力学研究中找到应用。

更新日期：2017-09-25

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