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Self-Assembly of Mesoscale Artificial Clathrin Mimics
ACS Nano ( IF 15.8 ) Pub Date : 2017-09-25 00:00:00 , DOI: 10.1021/acsnano.7b03739
Yifan Kong 1 , Mina-Elraheb S. Hanna 1 , Denys Zhuo 1 , Katherine G. Chang 1 , Tara Bozorg-Grayeli 1 , Nicholas A. Melosh 1
Affiliation  

Fluidic control and sampling in complex environments is an important process in biotechnology, materials synthesis, and microfluidics. An elegant solution to this problem has evolved in nature through cellular endocytosis, where the dynamic recruitment, self-assembly, and spherical budding of clathrin proteins allows cells to sample their external environment. Yet despite the importance and utility of endocytosis, artificial systems which can replicate this dynamic behavior have not been developed. Guided by clathrin’s unusual structure, we created simplified metallic microparticles that capture the three-legged shape, particle curvature, and interfacial attachment characteristics of clathrin. These artificial clathrin mimics successfully recreate biomimetic analogues of clathrin’s recruitment, assembly, and budding, ultimately forming extended networks at fluid interfaces and invaginating immiscible phases into spheres under external fields. Particle curvature was discovered to be a critical structural motif, greatly limiting irreversible aggregation and inducing the legs’ selective tip-to-tip attraction. This architecture provides a template for a class of active self-assembly units to drive structural and dimensional transformations of liquid–liquid interfaces and microscale fluidic sampling.

中文翻译:

中尺度人工网格蛋白模拟物的自组装。

复杂环境中的流体控制和采样是生物技术,材料合成和微流体学中的重要过程。通过细胞内吞作用,自然界已经解决了这个问题的一种绝妙解决方案,其中网格蛋白的动态募集,自组装和球形萌芽使细胞能够采样其外部环境。尽管具有内吞作用的重要性和实用性,但尚未开发出能够复制这种动态行为的人工系统。在网格蛋白的非常规结构的指导下,我们创建了简化的金属微粒,可捕获网格蛋白的三足形状,颗粒曲率和界面附着特性。这些人工网格蛋白模拟物成功地再现了网格蛋白募集,组装和萌芽的仿生类似物,最终在流体界面处形成扩展网络,并将不混溶相引入外部场下的球体中。发现粒子曲率是关键的结构图案,极大地限制了不可逆的聚集并诱导了腿部对头部的选择性吸引。该体系结构为一类主动自组装单元提供了模板,以驱动液-液界面和微观尺度流体采样的结构和尺寸转换。
更新日期:2017-09-26
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