Small Molecule Release and Activation through DNA Computing,Journal of the American Chemical Society

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Small Molecule Release and Activation through DNA Computing
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-09-25 , DOI: 10.1021/jacs.7b07831
Kunihiko Morihiro ₁ , Nicholas Ankenbruck ₁ , Bradley Lukasak ₁ , Alexander Deiters ₁

Affiliation

DNA-based logic gates can be assembled into computational devices that generate a specific output signal in response to oligonucleotide input patterns. The ability to interface with biological and chemical environments makes DNA computation a promising technology for monitoring cellular systems. However, DNA logic gate circuits typically provide a single-stranded oligonucleotide output, limiting the ability to effect biology. Here, we introduce a novel DNA logic gate design capable of yielding a small molecule output signal. Employing a Staudinger reduction as a trigger for the release and activation of a small molecule fluorophore, we constructed AND and OR logic gates that respond to synthetic microRNA (miRNA) inputs. Connecting the gates in series led to more complex DNA circuits that provided a small molecule output in response to a specific pattern of three different miRNAs. Moreover, our gate design can be readily multiplexed as demonstrated by simultaneous small molecule activation from two independent DNA circuits.

中文翻译：

通过 DNA 计算释放和激活小分子

基于 DNA 的逻辑门可以组装到计算设备中，这些设备会根据寡核苷酸输入模式生成特定的输出信号。与生物和化学环境交互的能力使 DNA 计算成为监测细胞系统的有前途的技术。然而，DNA 逻辑门电路通常提供单链寡核苷酸输出，限制了影响生物学的能力。在这里，我们介绍了一种能够产生小分子输出信号的新型 DNA 逻辑门设计。使用施陶丁格还原作为小分子荧光团释放和激活的触发器，我们构建了响应合成 microRNA (miRNA) 输入的 AND 和 OR 逻辑门。将门串联连接导致更复杂的 DNA 电路，提供小分子输出以响应三种不同 miRNA 的特定模式。此外，我们的门设计可以很容易地进行多路复用，正如来自两个独立 DNA 电路的同时小分子激活所证明的那样。

更新日期：2017-09-25

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