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Reduced Drought Tolerance by CRISPR/Cas9-Mediated SlMAPK3 Mutagenesis in Tomato Plants
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-09-25 00:00:00 , DOI: 10.1021/acs.jafc.7b02745 Liu Wang 1 , Lin Chen 1 , Rui Li 1 , Ruirui Zhao 1 , Meijing Yang 1 , Jiping Sheng 2 , Lin Shen 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-09-25 00:00:00 , DOI: 10.1021/acs.jafc.7b02745 Liu Wang 1 , Lin Chen 1 , Rui Li 1 , Ruirui Zhao 1 , Meijing Yang 1 , Jiping Sheng 2 , Lin Shen 1
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Drought stress is one of the most destructive environmental factors that affect tomato plants adversely. Mitogen-activated protein kinases (MAPKs) are important signaling molecules that respond to drought stress. In this study, SlMAPK3 was induced by drought stress, and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system was utilized to generate slmapk3 mutants. Two independent T1 transgenic lines and wild-type (WT) tomato plants were used for analysis of drought tolerance. Compared with WT plants, slmapk3 mutants exhibited more severe wilting symptom, higher hydrogen peroxide content, lower antioxidant enzymes activities, and suffered more membrane damage under drought stress. Furthermore, knockout of SlMAPK3 led to up- or down-regulated expressions of drought stress-responsive genes including SlLOX, SlGST, and SlDREB. The results suggest that SlMAPK3 is involved in drought response in tomato plants by protecting cell membranes from oxidative damage and modulating transcription of stress-related genes.
中文翻译：
通过CRISPR / Cas9介导的SlMAPK3诱变降低番茄植物的耐旱性
干旱胁迫是对番茄植物产生不利影响的最具破坏性的环境因素之一。丝裂原激活的蛋白激酶（MAPK）是重要的信号分子，可以响应干旱胁迫。在这项研究中，SlMAPK3是由干旱胁迫诱导的，并且利用簇状规则间隔的短回文重复序列（CRISPR）/ CRISPR相关蛋白9（CRISPR / Cas9）系统生成了slmapk3突变体。使用两个独立的T1转基因品系和野生型（WT）番茄植物来分析抗旱性。与野生植物相比，slmapk3突变体表现出更严重的萎症状，更高的过氧化氢含量，更低的抗氧化酶活性，并在干旱胁迫下遭受更多的膜破坏。此外，SlMAPK3的敲除导致干旱胁迫响应基因（包括SlLOX，SlGST和SlDREBEB）的表达上调或下调。结果表明，SlMAPK3通过保护细胞膜免受氧化损伤并调节胁迫相关基因的转录而参与番茄植株的干旱反应。
更新日期：2017-09-26
中文翻译：
通过CRISPR / Cas9介导的SlMAPK3诱变降低番茄植物的耐旱性
干旱胁迫是对番茄植物产生不利影响的最具破坏性的环境因素之一。丝裂原激活的蛋白激酶（MAPK）是重要的信号分子，可以响应干旱胁迫。在这项研究中，SlMAPK3是由干旱胁迫诱导的，并且利用簇状规则间隔的短回文重复序列（CRISPR）/ CRISPR相关蛋白9（CRISPR / Cas9）系统生成了slmapk3突变体。使用两个独立的T1转基因品系和野生型（WT）番茄植物来分析抗旱性。与野生植物相比，slmapk3突变体表现出更严重的萎症状，更高的过氧化氢含量，更低的抗氧化酶活性，并在干旱胁迫下遭受更多的膜破坏。此外，SlMAPK3的敲除导致干旱胁迫响应基因（包括SlLOX，SlGST和SlDREBEB）的表达上调或下调。结果表明，SlMAPK3通过保护细胞膜免受氧化损伤并调节胁迫相关基因的转录而参与番茄植株的干旱反应。
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