In this study, the preparation and structural properties of spiral dextrin (SD)/vitamin E and SD/soy isoflavone inclusion complexes were studied. SD was obtained from debranched normal maize starch using isoamylases. After fractionation using a novel method of gradient ethanol precipitation, SD was separated into different fractions, among which SD-40 was found to be the optimal host molecule to prepare SD inclusion complexes with vitamin E or soy isoflavone. X-ray diffraction (XRD) and ¹³C cross-polarization magic angle spinning nuclear magnetic resonance (NMR) suggested that the crystalline structures of SD-40/vitamin E and SD-40/soy isoflavone were V6II and V6III types, respectively. Small-angle X-ray scattering revealed that the SD-40/vitamin E inclusion complex formed a tighter and more compact crystallite than the SD-40/soy isoflavone inclusion complex. Furthermore, the connection structures of inclusion complexes were investigated by two-dimensional nuclear Overhauser effect spectroscopy NMR, indicating that part of vitamin E with an alkyl chain was encapsulated in the helix cavity of SD-40, whereas the aromatic ring B of the soy isoflavone molecule was complexed by the helix cavity and screw of SD.

中文翻译：

---

螺旋糊精包合物与维生素E或大豆异黄酮的比较结构表征

在这项研究中，研究了螺旋糊精（SD）/维生素E和SD /大豆异黄酮包合物的制备及其结构性能。使用异淀粉酶从脱支的正常玉米淀粉获得SD。使用梯度乙醇沉淀的新方法进行分级分离后，将SD分为不同的级分，其中SD-40被发现是制备与维生素E或大豆异黄酮形成SD包合物的最佳宿主分子。X射线衍射（XRD）和¹³C交叉极化魔角旋转核磁共振（NMR）表明SD-40 /维生素E和SD-40 /大豆异黄酮的晶体结构分别为V6II和V6III型。小角度X射线散射显示，SD-40 /维生素E包合物比SD-40 /大豆异黄酮包合物更紧密，更致密。此外，通过二维核Overhauser效应核磁共振NMR研究了包合物的连接结构，表明部分具有烷基链的维生素E被包裹在SD-40的螺旋腔中，而大豆异黄酮的芳香环B分子被SD的螺旋腔和螺旋复合。