Hybrid nanogels have been widely used as multifunctional drug delivery carriers and imaging probes for biomedical applications. Two triblock artificial polypeptides PC₁₀A and PC₁₀ARGD were biosynthesized to prepare hybrid nanogels. When the concentration of these polypeptides drops to less than 2% (w/w), they can form nanogels by self-assembly. The physical characteristics of nanogels, such as surface potential, size, and targeting domain are able to be tuned. Polypeptide–gold nanoparticles hybrid nanogels were in situ synthesized using PC₁₀A(RGD) as templates and photoinitiator I-2959 under 365 nm UV light irradiation in one step. The results of the effect of gold ion concentration on synthesized gold nanoparticles in hybrid nanogels showed that the size and the concentration of gold nanoparticles in hybrid nanogel increased gradually with the increasing of gold ion concentration. The concentration of polypeptide has no obvious effect on the properties of gold nanoparticles in hybrid nanogels and only influences the size of the hybrid nanogels. The concentration of gold nanoparticles in hybrid nanogels increased with the increasing of irradiation time. In addition, the change of pH (3.0–7.0) did not affect the properties of the gold nanoparticles in the hybrid nanogels. Cytotoxicity results showed that hybrid nanogels were almost nontoxic to HeLa cells when the concentration of Au ion was below 0.72 mM. An arginine-glycine-aspartic acid motif could be introduced into the PC₁₀ARGD–gold nanoparticles hybrid nanogels to enhance efficient receptor-mediated endocytosis in α_vβ₃ overexpressing HeLa cells as analyzed by photoacoustic imaging. These results indicate that such hybrid nanogels are promising to be used in biomedical applications.

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一步一步合成多肽-金纳米颗粒杂化纳米凝胶及其在靶向光声成像中的应用

杂合纳米凝胶已被广泛用作生物医学应用的多功能药物递送载体和成像探针。生物合成两种三嵌段人工多肽PC ₁₀ A和PC ₁₀ ARGD，以制备杂化纳米凝胶。当这些多肽的浓度下降到小于2％（w / w）时，它们可以通过自组装形成纳米凝胶。可以调整纳米凝胶的物理特性，例如表面电势，大小和靶向区域。使用PC ₁₀原位合成多肽-金纳米颗粒杂化纳米凝胶一步以A（RGD）为模板和光引发剂I-2959在365 nm UV光照射下进行。金离子浓度对杂化纳米凝胶中合成的金纳米颗粒的影响结果表明，杂化纳米凝胶中金纳米颗粒的大小和浓度随着金离子浓度的增加而逐渐增加。多肽的浓度对杂化纳米凝胶中金纳米颗粒的性质没有明显影响，仅影响杂化纳米凝胶的大小。杂化纳米凝胶中金纳米颗粒的浓度随着辐照时间的增加而增加。此外，pH值（3.0-7.0）的变化不会影响杂化纳米凝胶中金纳米颗粒的性能。细胞毒性结果显示，当Au离子浓度低于0.72 mM时，杂合纳米凝胶对HeLa细胞几乎无毒。可以将精氨酸-甘氨酸-天冬氨酸基序引入PC₁₀ ARGD金纳米颗粒混合纳米凝胶，以提高效率的受体介导的胞吞作用在α _v β _3个过表达HeLa细胞通过光声成像仪分析。这些结果表明，这种杂化纳米凝胶有望用于生物医学应用。