Multifrequency Optomechanical Stiffness Measurement of Single Adherent Cells on a Solid Substrate with High Throughput,Analytical Chemistry

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Multifrequency Optomechanical Stiffness Measurement of Single Adherent Cells on a Solid Substrate with High Throughput
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-25 00:00:00 , DOI: 10.1021/acs.analchem.7b02356
Ali Mehrnezhad ₁ , Kidong Park ₁

Affiliation

Mechanical properties of a cell reflect its biological and pathological conditions and there have been active research efforts to develop high-throughput platforms to mechanically characterize single cells. Yet, many of these research efforts are focused on suspended cells and use a flow-through configuration. In this paper, the stiffness of single adherent cells are optomechanically characterized using the vibration-induced phase shift (VIPS) without detaching them from the substrate. With the VIPS measurements, the frequency and amplitude dependency of the cell stiffness is investigated and statistically significant difference in the cell stiffness is confirmed after exposure to various drugs affecting cytoskeleton network. Furthermore, a 3-dimensional finite element model of a cell on a vibrating substrate is developed to extract the mechanical property from the measured VIPS. The developed technique can characterize the mechanical properties of single adherent cells at multiple frequencies with high throughput and will provide valuable clues in understanding cell mechanics.

中文翻译：

高通量固体基质上单个贴壁细胞的多频光机械刚度测量

细胞的机械性质反映了其生物学和病理状况，并且已经进行了积极的研究努力以开发高通量平台来机械表征单个细胞。然而，许多这些研究工作都集中在悬浮细胞上，并使用流通结构。在本文中，使用振动诱导相移（VIPS）进行光机械表征单个贴壁细胞的刚度，而不将其从基底上剥离。通过VIPS测量，研究了细胞刚度的频率和幅度依赖性，并在暴露于影响细胞骨架网络的各种药物后，证实了细胞刚度的统计学显着性差异。此外，建立了振动基板上单元的三维有限元模型，以从测量的VIPS中提取机械性能。所开发的技术可以高通量表征多个频率下的单个贴壁细胞的机械性能，并将为理解细胞力学提供有价值的线索。

更新日期：2017-09-25

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