The project aim was to identify differences in the metabolomic profiles in the serum of patients with multiple sclerosis (MS), those with neuropathic pain (NP) and those with both MS and NP compared with controls and to identify potential biomarkers of each disease state. Metabolomic profiling was performed using ultra-high-performance liquid chromatography coupled to mass spectrometry and the data analysis involved parametric methods, principal component analysis, and discriminating filter analysis to determine the differences between disease and control serum samples. Sphingosine and dihydrosphingosine were identified as significant biomarkers.

中文翻译：

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鞘氨醇和二氢鞘氨醇作为多发性硬化症的生物标志物，通过使用偶联的UPLC-MS的代谢组学分析鉴定

该项目的目的是与对照相比，鉴定多发性硬化症（MS），神经性疼痛（NP）以及MS和NP两者的患者血清中的代谢组学谱差异，并鉴定每种疾病状态的潜在生物标志物。使用结合质谱的超高效液相色谱进行代谢组学分析，数据分析涉及参数方法，主成分分析和区分过滤器分析，以确定疾病样本与对照血清样本之间的差异。鞘氨醇和二氢鞘氨醇被鉴定为重要的生物标记。