“Open-top” microfluidic device for in vitro three-dimensional capillary beds,Lab on a Chip

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“Open-top” microfluidic device for in vitro three-dimensional capillary beds
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-09-08 00:00:00 , DOI: 10.1039/c7lc00646b
Soojung Oh _{1,

2,

3,

4,

5} , Hyunryul Ryu _{6,

7,

8,

9} , Dongha Tahk _{1,

2,

3,

4} , Jihoon Ko _{1,

2,

3,

4} , Yoojin Chung _{4,

10,

11,

12} , Hae Kwang Lee _{4,

5,

12} , Tae Ryong Lee _{4,

5,

12} , Noo Li Jeon _{1,

2,

3,

4,

13}

Affiliation

We introduce a novel microfluidic device to co-culture a blood vessel network and cell tissues in an in vivo-like niche. Our “open-top” microfluidic device is composed of microchannels with micropores in the ceiling, which provides direct fluid access from reservoir to microchannel. Fluid connections through micropores afford novel advantages, including: i) the long-term culture of large-scale microvessel network, ii) access of different fluids to inner and exterior sides of the microvessel, and iii) co-culturing of the microvessel network and small cell tissue. In this study, we have successfully assembled microvessels with 5 mm channel widths. We were also able to mimic capillary bed conditions by co-culturing microvessels with cancer spheroids. Intimate contact between the cancer spheroid and microvessel caused vessel recruitment and an increase in vessel formation, and affected vessel morphology. We expect this device to be used as a novel platform for vascularized tissue models.

中文翻译：

用于体外三维毛细血管床的“开放式”微流体装置

我们介绍一种新型的微流控设备，以在体内共培养血管网络和细胞组织像利基。我们的“开顶式”微流体装置由在顶部具有微孔的微通道组成，可提供从储层到微通道的直接流体通道。通过微孔的流体连接具有新颖的优势，包括：i）大型微血管网络的长期培养，ii）不同的流体进入微血管的内部和外部，iii）微血管网络的共培养和小细胞组织。在这项研究中，我们成功地组装了5 mm通道宽度的微血管。我们还能够通过将微血管与癌症球体共同培养来模拟毛细血管床状况。癌症球体和微血管之间的紧密接触导致血管募集和血管形成增加，并影响血管形态。

更新日期：2017-09-25

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