A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity,Analyst

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A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity
Analyst ( IF 3.6 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1039/c7an00611j
Abu Ali Ibn Sina _{1,

2,

3,

4} , Matthew Thomas Foster _{1,

2,

3,

4} , Darren Korbie _{1,

2,

3,

4} , Laura G. Carrascosa _{1,

2,

3,

4} , Muhammad J. A. Shiddiky _{1,

2,

3,

4} , Jing Gao _{1,

2,

3,

4} , Shuvashis Dey _{1,

2,

3,

4} , Matt Trau _{1,

2,

3,

4,

5}

Affiliation

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl⁻¹ of DNA with no sequencing requirement.

中文翻译：

利用金-DNA亲和力检测多种DNA甲基化事件的多重微平台

我们报告了跨多个区域的区域DNA甲基化的电化学检测的新的多重策略。使用亚硫酸氢盐处理过的DNA对金表面的序列依赖性亲和力，该方法将包括金电极微阵列的微型多重设备的高灵敏度与多重PCR的强大多重能力整合在一起。这种组合的协同作用使得能够同时从低至500 pgμl ^-1的DNA监测多个基因组区域的甲基化变化，而无需测序。

更新日期：2017-09-25

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