An electrochemiluminescence cloth-based biosensor with smartphone-based imaging for detection of lactate in saliva,Analyst

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An electrochemiluminescence cloth-based biosensor with smartphone-based imaging for detection of lactate in saliva
Analyst ( IF 3.6 ) Pub Date : 2017-08-14 00:00:00 , DOI: 10.1039/c7an01008g
Yong Yao _{1,

2,

3,

4,

5} , Huijie Li _{1,

2,

3,

4,

5} , Dan Wang _{1,

2,

3,

4,

5} , Cuiling Liu _{1,

2,

3,

4,

5} , Chunsun Zhang _{1,

2,

3,

4,

5}

Affiliation

Cloth fabrics and smartphones have become the two things people are most familiar with. Particularly, smartphones are used as portable personal computers, revolutionizing communication and lifestyle. Here, screen-printing technology is applied to fabricate carbon electrodes and electrochemical chambers on a single hydrophilic cloth, while the cloth-based electrochemiluminescence (ECL) signals are read out by using an inexpensive smartphone. Therefore, the ECL detection is available in both low-cost disposable sensors and a portable format, which may be very suitable to be used as a non-invasive monitoring tool for medical diagnostics. As a proof-of-principle, lactate oxidase is immobilized onto the working electrode for the lactate measurement. Under optimized conditions, the lactate levels can be quantified over the range of 0.05–2.5 mM, with a detection limit of 0.035 mM and the relative standard deviations of 4.7%, 5.2% and 5.0% for 0.05, 0.5 and 2.5 mM lactate (n = 5). In addition, the proposed biosensor has an acceptable stability and selectivity. Finally, the ECL biosensor is successfully applied for the determination of lactate in human saliva. These results show that the presented ECL platform has the potential to be applied to non-invasively detect a variety of analytes of medical interest.

中文翻译：

基于电化学发光布的生物传感器和基于智能手机的成像技术，可检测唾液中的乳酸

布面料和智能手机已成为人们最熟悉的两件事。特别地，智能电话被用作便携式个人计算机，彻底改变了通信和生活方式。在这里，丝网印刷技术被用于在单块亲水性布料上制造碳电极和电化学室，同时使用廉价的智能手机读取基于布料的电化学发光（ECL）信号。因此，ECL检测既可以使用低成本的一次性传感器，也可以使用便携式格式，这可能非常适合用作医疗诊断的非侵入性监视工具。作为原理证明，将乳酸氧化酶固定在工作电极上以进行乳酸测量。在最佳条件下，乳酸水平可以在0.05–2.5 mM的范围内进行定量，n = 5）。另外，提出的生物传感器具有可接受的稳定性和选择性。最终，ECL生物传感器成功应用于人唾液中乳酸的测定。这些结果表明，提出的ECL平台具有潜力，可用于非侵入性地检测医学上感兴趣的各种分析物。

更新日期：2017-09-25

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