Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS,Analyst

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Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS
Analyst ( IF 3.6 ) Pub Date : 2017-08-08 00:00:00 , DOI: 10.1039/c7an00954b
Qi Wang _{1,

2,

3,

4,

5} , Keyun Wang _{1,

2,

3,

4,

5} , Mingliang Ye _{1,

2,

3,

4,

5}

Affiliation

Protein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at the proteome level is still a great challenge. The alteration of the protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate methylated peptides from a pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we report on recent advances in the development of methods for large-scale analysis of non-histone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence have been covered.

中文翻译：

LC-MS / MS大规模分析非组蛋白甲基化的策略

蛋白质甲基化是重要的翻译后修饰（PTM），在多种生物过程的调控中起着至关重要的作用。尽管已经为蛋白质甲基化的研究做出了很多努力，但是在蛋白质组水平上对非组蛋白甲基化的分析仍然是一个巨大的挑战。由甲基化引起的蛋白质/肽物理化学性质的变化非常小，因此很难开发出高效的富集方法来从多种背景肽库中分离出甲基化肽。由甲基化引起的质量转移与某些氨基酸的取代相同，因此很难确定性地鉴定甲基化的肽。在这篇评论中，我们报告了非组蛋白甲基化大规模分析方法的最新进展。特别地，已经涵盖了有效富集的方法和控制识别置信度的方法。

更新日期：2017-09-25

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