Raman endoscopy for monitoring the anticancer drug treatment of colorectal tumors in live mice,Analyst

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Raman endoscopy for monitoring the anticancer drug treatment of colorectal tumors in live mice
Analyst ( IF 3.6 ) Pub Date : 2017-08-07 00:00:00 , DOI: 10.1039/c7an00720e
Akinori Taketani _{1,

2,

3,

4,

5} , Bibin B. Andriana _{1,

2,

3,

4,

5} , Hiroko Matsuyoshi _{1,

2,

3,

4,

5} , Hidetoshi Sato _{1,

2,

3,

4,

5}

Affiliation

A miniaturized Raman endoscope (mRE) system was employed to study the effects of anticancer treatment on colorectal tumors in a live murine model. The endoscope is narrow enough to observe the inside of the mouse colon under anesthesia. It has a channel for a ball lens mounted on a hollow fiber Raman probe (BHRP) to measure any targeted point under the visual control of the endoscope. Colorectal cancer tissue was observed to study the alterations of the tissue in response to anticancer drug treatment. Three anticancer drugs, 5-fluorouracil (5-FU), cisplatin (CDDP), and docetaxel, were employed. Although no alteration was recognized in the endoscopic visual observations at 2 weeks after the drug treatment, the Raman spectra obtained in the live mouse colon indicated that molecular changes of lipids and proteins were observed. This study demonstrates that in situ Raman analysis is highly sensitive for detecting the effects of anticancer drugs.

中文翻译：

拉曼内窥镜监测活小鼠大肠肿瘤的抗癌药物治疗

微型拉曼内窥镜（mRE）系统用于研究活鼠模型中抗癌治疗对结直肠肿瘤的影响。内窥镜足够窄，可以在麻醉下观察小鼠结肠内部。它有一个球镜通道，该球镜安装在中空纤维拉曼探针（BHRP）上，可以在内窥镜的视觉控制下测量任何目标点。观察结直肠癌组织以研究响应抗癌药物治疗的组织变化。使用了三种抗癌药：5-氟尿嘧啶（5-FU），顺铂（CDDP）和多西他赛。尽管在药物治疗后2周的内窥镜视觉观察中没有发现变化，但是在活小鼠结肠中获得的拉曼光谱表明观察到了脂质和蛋白质的分子变化。原位拉曼分析对于检测抗癌药物的效果非常敏感。

更新日期：2017-09-25

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