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Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1039/c7sc02819a Cheng Jin 1, 2, 3, 4, 5 , Ting Fu 1, 2, 3, 4, 5 , Ruowen Wang 1, 2, 3, 4, 5 , Hui Liu 1, 2, 3, 4, 5 , Jianmei Zou 1, 2, 3, 4, 5 , Zilong Zhao 1, 2, 3, 4, 5 , Mao Ye 1, 2, 3, 4, 5 , Xiaobing Zhang 1, 2, 3, 4, 5 , Weihong Tan 1, 2, 3, 4, 5
Chemical Science ( IF 7.6 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1039/c7sc02819a Cheng Jin 1, 2, 3, 4, 5 , Ting Fu 1, 2, 3, 4, 5 , Ruowen Wang 1, 2, 3, 4, 5 , Hui Liu 1, 2, 3, 4, 5 , Jianmei Zou 1, 2, 3, 4, 5 , Zilong Zhao 1, 2, 3, 4, 5 , Mao Ye 1, 2, 3, 4, 5 , Xiaobing Zhang 1, 2, 3, 4, 5 , Weihong Tan 1, 2, 3, 4, 5
Affiliation
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Molecular beacons (MBs) are simple, but practical, fluorescent nanoprobes widely used to detect small molecules, nucleic acids and proteins. However, some challenges still remain when MBs are employed in complex biological environments, such as instability and non-target interference. To meet such challenges, we have designed and synthesized fluorinated molecular beacons (FMBs) as functional DNA nanomolecules for cellular imaging, in which the stem sequence is simply composed of artificial nucleotides with 3,5-bis(trifluoromethyl)benzene (F) as the surrogate base of natural A, T, C and G bases. The introduction of F base into MBs significantly increases their hydrophobicity, and the stem is formed by the assembly of self-complementary base F nucleotides through hydrophobic interactions. Fluorescence studies revealed that FMBs confer improved stability over conventional MBs. To demonstrate the application of FMBs for cellular imaging, we constructed an FMB to detect mRNA in MCF-7 cells, and the FMB was proven to be a practical nanoprobe for cellular imaging of mRNA.
中文翻译：
氟化分子信标作为细胞成像的功能性DNA纳米分子
分子信标（MBs）是一种简单但实用的荧光纳米探针，广泛用于检测小分子，核酸和蛋白质。但是，在复杂的生物环境中使用MB时，仍然存在一些挑战，例如不稳定和非目标干扰。为了应对这些挑战，我们设计并合成了氟化分子信标（FMBs）作为用于细胞成像的功能性DNA纳米分子，其中茎序列仅由人工核苷酸组成，其中人工核苷酸为3,5-双（三氟甲基）苯（F）。替代天然A，T，C和G碱基。将F碱基引入MBs显着增加了它们的疏水性，并且茎是通过疏水相互作用通过自身互补碱基F核苷酸的组装而形成的。荧光研究表明，与传统的MB相比，FMB具有更高的稳定性。为了演示FMB在细胞成像中的应用，我们构建了FMB来检测MCF-7细胞中的mRNA，并且事实证明FMB是用于mRNA细胞成像的实用纳米探针。
更新日期：2017-09-25
中文翻译：
氟化分子信标作为细胞成像的功能性DNA纳米分子
分子信标（MBs）是一种简单但实用的荧光纳米探针，广泛用于检测小分子，核酸和蛋白质。但是，在复杂的生物环境中使用MB时，仍然存在一些挑战，例如不稳定和非目标干扰。为了应对这些挑战，我们设计并合成了氟化分子信标（FMBs）作为用于细胞成像的功能性DNA纳米分子，其中茎序列仅由人工核苷酸组成，其中人工核苷酸为3,5-双（三氟甲基）苯（F）。替代天然A，T，C和G碱基。将F碱基引入MBs显着增加了它们的疏水性，并且茎是通过疏水相互作用通过自身互补碱基F核苷酸的组装而形成的。荧光研究表明，与传统的MB相比，FMB具有更高的稳定性。为了演示FMB在细胞成像中的应用，我们构建了FMB来检测MCF-7细胞中的mRNA，并且事实证明FMB是用于mRNA细胞成像的实用纳米探针。
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